eoe sub articles

EoE Sub Articles

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board
1. Solutions Required
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	<li>Prepare 150 mg/L (for anesthesia) or 300 mg/L (for euthanasia) of ethyl 3-aminobenzoate methanesulfonate (MS-222) solution with water from the aquarium where the zebrafish are maintained. Fill a small tank with 1 L of anesthetic solution and keep it aerated.</li>
	<li>Fill another small tank with 1 L o...

oral intubation of adult zebrafish to administer experimental compounds

Source: Ji, J., Thwaite, R. et al. <a href="https://app.jove.com/v/58366">Oral Intubation of Adult Zebrafish: A Model for Evaluating Intestinal Uptake of Bioactive Compounds.</a> J. Vis. Exp. (2018).
This video demonstrates a technique for orally intubating adult zebrafish to administer experimental compounds into the esophagus and monitor their intestinal uptake. This method offers a valuable model for studying the dynamics of oral prophylactics or other compounds within the intestine.

Oral Intubation of Adult Zebrafish to Administer Experimental Compounds

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board
1. ...

Begin by loading a sterile oral intubation device with a suspension of nanostructured cytokine inclusion bodies&#160; &#8212; the experimental nanoparticle.
Place a fasting anesthetized zebrafish onto a moist plastic tray.
Hold the fish steadily and gently open its mouth using the pipette tip attached to the oral intubation device.
Carefully insert the needle through the fish&#39;s mouth, down the esophagus.
Once the pipette tip passes the gills and encounters a slight resistance, avoid further insertion.
Gently inject the nanoparticles into the fish.
Upon injection, these cytokine nanoparticles come in direct contact with the epithelial cells that line the digestive tract of the zebrafish.
These cells internalize the nanoparticles through endocytosis.
Within the epithelial cell, the vesicle undergoes intracellular processing to release the cytokine nanoparticles.
These cytokine nanoparticles exit into the underlying connective tissue, interact with resident immune cells, and trigger intracellular signaling events, resulting in a cytokine-driven immune response.