eoe sub articles

EoE Sub Articles

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Anesthetize, Intubate, and Inoculate Animals
NOTE: Specific pathogen-free (SPF) immunocompetent male Sprague-Dawley rats weighing 100 - 120 g are recommended. Provide all animals with food and water ad libitum and house them socially on wood chips or other absorbent bedding with s...

intrabronchial instillation of streptococcus pneumoniae in immunocompetent rodent models

Source: Hoover, J. L., et al., <a href="https://app.jove.com/v/55068">A Robust Pneumonia Model in Immunocompetent Rodents to Evaluate Antibacterial Efficacy against S. pneumoniae, H. influenzae, K. pneumoniae, P. aeruginosa or A. baumannii.</a> J. Vis. Exp. (2017)
This video demonstrates the establishment of lung infection in an immunocompetent rodent model via intubation and installation of bacterial suspensions into lung lobes. This model enables antibacterial efficacy studies in immunocompetent animals.

<img alt="Figure 1" src="/files/ftp_upload/21758/21758_Figure1.jpg" /> 
Figure 1: Nonsurgical Intratracheal Intubation. Tools are shown for intubating the rat (A) and the mouse (B). Once anesthetized, the animal should be positioned as shown in C, with the head to the right and tail to the left if the individual performing the technique is right-handed. Place the left forefinger gently on the throat ...

Intrabronchial Instillation of Streptococcus pneumoniae in Immunocompetent Rodent Models

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. ...

Obtain a sterile intubation device consisting of a metal cannula with polyethylene tubing connected to a metal needle.
Attach a syringe containing Streptococcus pneumoniae to the needle, and fill the tubing.
Next, position an anesthetized immunocompetent rat in a supine position.
Insert the free end of the metal cannula into the rat&#39;s mouth. Advance it into the trachea toward the left bronchus and down the left lung to an appropriate depth.
Advance the tubing to the end of the cannula and instill the bacterial suspension into the left lung lobe.
The bacterial capsule and surface-associated enzymes prevent bacterial entrapment in the mucus. Further, Streptococcus pneumoniae attach to the surface receptors on bronchial epithelial cells, facilitating endocytosis and release to the basolateral surface.&#160;
Bacteria secrete toxins that damage the epithelial cells and barriers, triggering an immune response.
However, Streptococcus pneumoniae has developed strategies to evade the host immune response and establish lung infection.