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Intrabronchial Instillation of Streptococcus pneumoniae in Immunocompetent Rodent Models
In This Article
Overview
This video demonstrates the establishment of lung infection in an immunocompetent rodent model via intubation and installation of bacterial suspensions into lung lobes. This model enables antibacterial efficacy studies in immunocompetent animals.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Anesthetize, Intubate, and Inoculate Animals
NOTE: Specific pathogen-free (SPF) immunocompetent male Sprague-Dawley rats weighing 100 - 120 g are recommended. Provide all animals with food and water ad libitum and house them socially on wood chips or other absorbent bedding with standard 12-hour light-dark cycles.
- Prior to conducting any experiments, obtain and sterilize a metal cannula and polyethylene tubing of ....
Representative Results
Figure 1: Nonsurgical Intratracheal Intubation. Tools are shown for intubating the rat (A) and the mouse (B). Once anesthetized, the animal should be positioned as shown in C, with the head to the right and tail to the left if the individual performing the technique is right-handed. Place the left forefinger gently on the throat .......
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Source: Hoover, J. L., et al., A Robust Pneumonia Model in Immunocompetent Rodents to Evaluate Antibacterial Efficacy against S. pneumoniae, H. influenzae, K. pneumoniae, P. aeruginosa or A. baumannii. J. Vis. Exp. (2017)
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