JoVE Logo
Faculty Resource Center

Sign In

JoVE Encyclopedia of Experiments

Overview

Protocol

Representative Results

Encyclopedia of Experiments

Intrabronchial Instillation of Streptococcus pneumoniae in Immunocompetent Rodent Models

This video demonstrates the establishment of lung infection in an immunocompetent rodent model via intubation and installation of bacterial suspensions into lung lobes. This model enables antibacterial efficacy studies in immunocompetent animals.

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Anesthetize, Intubate, and Inoculate Animals

NOTE: Specific pathogen-free (SPF) immunocompetent male Sprague-Dawley rats weighing 100 - 120 g are recommended. Provide all animals with food and water ad libitum and house them socially on wood chips or other absorbent bedding with s.......

Log in or to access full content. Learn more about your institution’s access to JoVE content here

Figure 1
Figure 1: Nonsurgical Intratracheal Intubation. Tools are shown for intubating the rat (A) and the mouse (B). Once anesthetized, the animal should be positioned as shown in C, with the head to the right and tail to the left if the individual performing the technique is right-handed. Place the left forefinger gently on the throat .......

Log in or to access full content. Learn more about your institution’s access to JoVE content here

This article has been published

Video Coming Soon

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved