eoe sub articles

EoE Sub Articles

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Assessment of Dry Eye Features
Measure corneal damage with fluorescein staining.
<ol>
	<li>Add 2 &#181;L of 0.2% fluorescein to the rat cornea. Passively open and close the rat eyelids 3 times with a gloved finger to spread the fluorescein dye on the surface of the eye.</li>
	<li>After...

fluorescein staining in an autoimmune dry eye rat model to examine corneal damage

Source: Hou Ahiua, et al. <a href="https://app.jove.com/v/55592">A Chronic Autoimmune Dry Eye Rat Model with Increase in Effector Memory T Cells in Eyeball Tissue.</a> J.Vis. Exp. (2017)
This video demonstrates the staining of the damaged cornea using fluorescein dye to evaluate the extent of autoimmune dry eye disease in a rat model. The green fluorescence spots of the stained collagen fibers indicate corneal epithelial damaged cells.

Fluorescein Staining in an Autoimmune Dry Eye Rat Model to Examine Corneal Damage

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. ...

Start with an anesthetized rat presenting symptoms of autoimmune dry eye disease.
Apply an appropriate amount of fluorescein dye over the rat&#39;s cornea.
Close and open the rat&#39;s eyelid to ensure the fluorescein spreads evenly over the cornea.
Allow the dye to settle and adhere to the cornea.
In autoimmune dry eye disease, the immune cells mistakenly target and attack the lacrimal glands, decreasing tear production.
This leads to the desiccation of the corneal epithelium and damage to the underlying Bowman&#39;s membrane &#8212; an acellular zone anterior to the stroma.
The fluorescein molecules permeate through the damaged corneal barriers to reach the underlying collagen-rich stromal layer.
Here, the fluorescein molecules bind to collagen, resulting in the staining of the collagen fibers.
Using an appropriate imaging technique, acquire images of the stained cornea.
Analyze the number, area, and intensity of the green fluorescent spots to measure the extent of corneal damage.