eoe sub articles

EoE Sub Articles

1. C. albicans Growth
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	<li>Twenty-four hours before the initiation of the animal experiment, prepare yeast extract peptone dextrose, YPD plates by adding 10 g of yeast extract granulated, 20 g of bacteriological peptone, and 15 g of granulated agar. Makeup volume to 900 ml with Milli-Q water and autoclave.</li>
	<li>Add 50 ml of sterile 40% glucose. Mix thoroughly and pour into Petri dishes. Leave agar plates to cool and solidify. 
	NOTE: In this study, use two strai...

candida albicans biofilm formation in an in vivo mouse model

Source: Kuchar&#237;kov&#225;, S., et al.&#160; <a href="https://app.jove.com/v/52239">Candida albicans Biofilm Development on Medically-relevant Foreign Bodies in a Mouse Subcutaneous Model Followed by Bioluminescence Imaging.</a> J. Vis. Exp. (2015).
This video demonstrates the development of an in vivo mouse model to study the biofilm formation of Candida albicans in subcutaneous tissue. The experiment involves implanting Candida-infected devices in the animal&#39;s back region for biofilm development.

<img alt="Figure 1" src="/files/ftp_upload/21797/21797_Figure1 .jpg" /> 
Figure 1: Major steps during the animal surgery. (A) The procedure of catheter implant. (1) Place the anesthetized animal on a warm pad containing paper tissue and apply the ophthalmic ointment to the eyes. (2) Shave the lower part of the back and disinfect. (3) Create two small (approx. 0.5 cm) incisions through the skin on the left and on the right side of the back. Create a subcutaneous tunne...

Candida albicans Biofilm Formation in an In Vivo Mouse Model

1. C. albicans Growth

	Twenty-four hours before the initiation of the animal experiment, prepare yeast extract peptone dextrose, YPD plates by adding 10 ...

Take a serum-coated catheter piece in a microcentrifuge tube.
Add Candida albicans, a common biofilm-producing fungal pathogen.
Incubate to allow interactions between surface adhesins on the fungal cells and serum proteins on the catheter, enabling fungal adherence.
Wash the catheter with a buffer to remove loosely attached cells.
Next, prepare an anesthetized immunocompromised mouse for surgery.
Make a small incision in the skin and create a subcutaneous tunnel.
Insert a catheter piece, previously infected with fungi, into the tunnel.
Suture the incision.
The fungal cells divide, forming microcolonies on the catheter. These microcolonies secrete an extracellular polymeric substance, or EPS&#160; matrix, promoting cell cohesion and surface adhesion.
EPS, a biomolecule-rich material, provides structural integrity to the biofilm and shields the microbes from the host&#39;s natural defenses.
Over time, the fungal biofilm develops a complex three-dimensional structure with channels and water-filled voids, enabling nutrient exchange, waste removal, and cell-to-cell communication.