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Encyclopedia of Experiments

Induction of Necrotizing Enterocolitis in Human Epithelial Enteroid Model

This video demonstrates the development of necrotizing enterocolitis disease in a human-derived neonatal enteroid model induced by lipopolysaccharides treatment. This model helps to study intestinal pathophysiology.

All procedures involving human participants have been performed in compliance with the institutional, national, and international guidelines for human welfare and have been reviewed by the local institutional review board.

1. Reagent Preparation

  1. Prepare Culture Media stock solution for whole tissue collection: 1 L of Dulbecco's Modified Eagle Medium (DMEM), 110 mL of Fetal Bovine Serum (FBS), 11 mL of Penicillin-Streptomycin (final concentration 1%), and 1.1 mL.......

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Source: Ares, G. J., et al. A Novel Human Epithelial Enteroid Model of Necrotizing Enterocolitis. J. Vis. Exp.(2019).

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