eoe sub articles

EoE Sub Articles

All procedures involving human participants have been performed in compliance with the institutional, national, and international guidelines for human welfare and have been reviewed by the local institutional review board.
1. Reagent Preparation
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	<li>Prepare Culture Media stock solution for whole tissue collection: 1 L of Dulbecco&#39;s Modified Eagle Medium (DMEM), 110 mL of Fetal Bovine Serum (FBS), 11 mL of Penicillin-Streptomycin (final concentration 1%), and 1.1 mL...

induction of necrotizing enterocolitis in human epithelial enteroid model

Source: Ares, G. J., et al. <a href="https://app.jove.com/v/59194">A Novel Human Epithelial Enteroid Model of Necrotizing Enterocolitis.</a> J. Vis. Exp.(2019).
This video demonstrates the development of necrotizing enterocolitis disease in a human-derived neonatal enteroid model induced by lipopolysaccharides treatment. This model helps to study intestinal pathophysiology.

Induction of Necrotizing Enterocolitis in Human Epithelial Enteroid Model

All procedures involving human participants have been performed in compliance with the institutional, national, and international guidelines for human ...

Obtain human small intestinal tissue-derived crypts &#160;&#8212; tubular invaginations containing stem cells and various types of epithelial cells.
Add the intestinal crypts into a chilled basement matrix.
Transfer this mixture into a multiwell plate, creating a three-dimensional dome.
Add growth medium and incubate.
Stem cells self-organize, proliferate, and differentiate into multiple types of intestinal cells, forming enterospheres.
Over time, the enterospheres mature into enteroids &#160;&#8211; three-dimensional self-renewing structures, resembling the intestinal crypts.
Add lipopolysaccharides, or LPS, a bacterial endotoxin, into the enteroid-containing well and incubate.
LPS molecules bind to the toll-like receptor in the enteroid, initiating a pro-inflammatory response, resulting in the accumulation of reactive oxygen species. This triggers a cascade of events leading to apoptosis.
Consequently, the integrity of the enteroid is compromised, leading to the penetration of LPS into the lumen, further intensifying the inflammatory response.
This mimics the development of necrotizing&#160; enterocolitis, a severe inflammation in the intestines of premature infants.