eoe sub articles

EoE Sub Articles

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Antigen preparation for subcutaneous injection
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	<li>Perform all procedures in this section inside a chemical fume hood to prevent inhalation or skin contact with the Mycobacterium tuberculosis (Mtb) H37Ra powder. Handle according to your institutional policies for Complete Freund&#39;s Adjuvant (typically BSL-1). Th...

optical coherence tomography imaging for assessing uveitis in a mouse model

Source: John, S., et al. <a href="https://app.jove.com/v/62925">Primed Mycobacterial Uveitis (PMU) as a Model for Post-Infectious Uveitis.</a> J. Vis. Exp. (2021)
This video demonstrates an in vivo imaging technique for detecting and quantifying eye inflammation in a mouse model of post-infectious uveitis. The technique, known as optical coherence tomography (OCT), generates high-resolution cross-sectional images of the eye for assessing disease progression.

<img alt="Figure 1" src="/files/ftp_upload/21807/21807_Figure1.jpg" /> 
Figure 1: Mouse intravitreal injection setup. The intravitreal injection is performed on the mouse eye using (A) a micro syringe pump controller connected to (B) the Micropump and (C) an injection syringe. The syringe is loaded and mounted on the (D) Micropump. The mouse head is positioned using (E) ear bars to e...

Optical Coherence Tomography Imaging for Assessing Uveitis in a Mouse Model

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. ...

Take an anesthetized mouse pre-immunized with heat-killed Mycobacterium tuberculosis, or Mtb, which induces an immune response, and generates Mtb-specific T cells.
Administer eye drops for pupil dilation to enhance light penetration for improved eye imaging. Apply a gel to prevent corneal dehydration.
Wrap the mouse in gauze to maintain body warmth and place it in the optical coherence tomography or OCT system.
Image the eyes to obtain cross-sectional images of the anterior chamber, including the cornea and lens, and the posterior chamber, including the retina.
Examine to ensure the absence of inflammation.
Inject heat-killed Mtb into the vitreous cavity. The immunogen spreads across the eye and triggers an immune response, inducing the infiltration of preformed Mtb-specific T cells and other immune cells, resulting in inflammation termed uveitis.
OCT of the anterior chamber reveals infiltrated immune cells and corneal edema, while OCT of the posterior chamber indicates vitreous inflammation.
Score OCT images based on inflammation severity to quantify uveitis progression.