JoVE Logo
Faculty Resource Center

Sign In

JoVE Encyclopedia of Experiments

Overview

Protocol

Encyclopedia of Experiments

Evaluation of Stromal Cytokine-Induced Intracellular Protein Phosphorylation Using Phospho-Flow Cytometry

This video demonstrates the evaluation of stromal cytokine-induced intracellular phosphorylation of proteins in leukemia cells. These intracellular phosphorylated proteins are quantified using the phospho-flow cytometry technique.

1. Phospho-flow Cytometry Assays to Evaluate the Activity of Cytokine Produced by Stroma

NOTE: Assess the supernatant from the engineered stroma before the first experiment to verify the relevant bio-activity of the stroma-generated cytokine for the individual experiment. Once the engineered stroma is validated, further testing is not necessary unless stroma are introduced that were produced with a new vector.

  1. Purchase MUTZ5 and/or MHH-CALL4 leukem.......

Log in or to access full content. Learn more about your institution’s access to JoVE content here

This article has been published

Video Coming Soon

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved