Agarose-Based Preparation of Human Precision-Cut Lung Slices

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Encyclopedia of Experiments

Agarose-Based Preparation of Human Precision-Cut Lung Slices

Overview

In this video, we describe a procedure to inflate isolated human lungs with agarose and prepare human precision-cut lung slices. The prepared ex vivo lung slices can be used for further immunologic experiments.

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Source: Neuhaus, V. et al., Assessment of the Cytotoxic and Immunomodulatory Effects of Substances in Human Precision-cut Lung Slices. J. Vis. Exp. (2018)