Assessment of the Cytotoxic and Immunomodulatory Effects of Substances in Human Precision-cut Lung Slices
13.6K Views
•
12:04 min
•
May 9th, 2018
DOI :
May 9th, 2018
•Vanessa Neuhaus*1, Olga Danov*1, Sebastian Konzok1, Helena Obernolte1, Susann Dehmel1, Peter Braubach2, Danny Jonigk2, Hans-Gerd Fieguth3, Patrick Zardo4, Gregor Warnecke4, Christian Martin5, Armin Braun1,6, Katherina Sewald1
1Fraunhofer Institute for Toxicology and Experimental Medicine (ITEM), German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Member of REBIRTH Cluster of Excellence, 2Institute for Pathology, Hannover Medical School, German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), 3Division of Thoracic and Vascular Surgery, Klinikum Region Hannover (KRH), 4Department of Cardiothoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), 5Institute of Pharmacology and Toxicology, RWTH Aachen University, 6Institute for Immunology, Hannover Medical School
Chapters in this video
0:05
Title
1:26
General Preparation of Human PCLS and Subsequent Exposure to Chemicals
4:28
Measurement of Chemically-induced Cytotoxicity in Human PCLS by WST-1 Assay
6:01
LDH Assay
7:29
Microscopic Viability Assay with Confocal Laser Scanning Microscope (cLSM)
9:49
Results: Representative Images of the Three-dimensional Microscopic Staining and Image Rendering of Human PCLS
10:59
Conclusion
Related Videos
09:50
Impact Assessment of Repeated Exposure of Organotypic 3D Bronchial and Nasal Tissue Culture Models to Whole Cigarette Smoke
11.1K Views
09:25
Methods to Evaluate Cytotoxicity and Immunosuppression of Combustible Tobacco Product Preparations
11.4K Views
09:33
Precision-cut Mouse Lung Slices to Visualize Live Pulmonary Dendritic Cells
16.9K Views
09:00
Characterization of Immune Cells and Proinflammatory Mediators in the Pulmonary Environment
3.3K Views
05:47
Generation of Human 3D Lung Tissue Cultures (3D-LTCs) for Disease Modeling
20.4K Views
10:10
Assessment of the Acute Inhalation Toxicity of Airborne Particles by Exposing Cultivated Human Lung Cells at the Air-Liquid Interface
6.9K Views
09:08
Precision Cut Lung Slices as an Efficient Tool for Ex vivo Pulmonary Vessel Structure and Contractility Studies
5.7K Views
08:59
Utilizing the Precision-Cut Lung Slice to Study the Contractile Regulation of Airway and Intrapulmonary Arterial Smooth Muscle
3.4K Views
01:20
Agarose-Based Preparation of Human Precision-Cut Lung Slices
316 Views
01:33
An Assay to Measure Chemically-Induced Cytotoxicity in Human Precision-Cut Lung Slices
168 Views
Copyright © 2025 MyJoVE Corporation. All rights reserved