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An Assay to Measure Chemically-Induced Cytotoxicity in Human Precision-Cut Lung Slices
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Overview
This video illustrates a technique for evaluating cytotoxicity in human precision-cut lung slices through the WST-1 assay. Human precision-cut lung slices are subjected to incremental concentrations of a cytotoxic chemical to quantify their cytotoxicity. A decrease in color intensity serves as confirmation of the chemical's detrimental impact on lung cells.
Protocol
All procedures involving sample collection have been performed in accordance with the institute's IRB guidelines.
1. General Preparation of Human precision-cut lung slices (PCLS) and Subsequent Exposure to Chemicals
NOTE: Two persons are required to fill a lung. An up-to-date vaccination record for hepatitis A and B is recommended. Patients are routinely screened for hepatitis C virus (HCV) and human immunodeficiency virus (HIV) p.......
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Source: Neuhaus, V. et al., Assessment of the Cytotoxic and Immunomodulatory Effects of Substances in Human Precision-cut Lung Slices. J. Vis. Exp. (2018)
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