An Assay to Measure Chemically-Induced Cytotoxicity in Human Precision-Cut Lung Slices

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Encyclopedia of Experiments

An Assay to Measure Chemically-Induced Cytotoxicity in Human Precision-Cut Lung Slices

Overview

This video illustrates a technique for evaluating cytotoxicity in human precision-cut lung slices through the WST-1 assay. Human precision-cut lung slices are subjected to incremental concentrations of a cytotoxic chemical to quantify their cytotoxicity. A decrease in color intensity serves as confirmation of the chemical's detrimental impact on lung cells.

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Source: Neuhaus, V. et al., Assessment of the Cytotoxic and Immunomodulatory Effects of Substances in Human Precision-cut Lung Slices. J. Vis. Exp. (2018)