eoe sub articles

EoE Sub Articles

All procedures involving human participants have been performed in compliance with the institutional, national, and international guidelines for human welfare and have been reviewed by the local institutional review board.
1. Instrument setup
NOTE: Imaging levitating cells requires two rare earth neodymium magnets magnetized on the z-axis to be placed with the same pole facing each other to generate a magnetic field. The distance betw...

detection of rhesus factor in human blood samples using magnetic levitation

Source: Thompson, L. et al., <a href="https://app.jove.com/v/62550">Quantification of Cellular Densities and Antigenic Properties using Magnetic Levitation.</a>&#160;J. Vis. Exp.&#160;(2021)
This video demonstrates the detection of the Rhesus (Rh) factor in blood samples using a magnetic levitation instrument. The magnetic field aligns and levitates beads and blood cells based on density. Treating the samples with anti-Rh antibodies results in intermediate-density bead-cell complexes&#39; formation, allowing for the differentiation of Rh-positive and Rh-negative red blood cells.

Detection of Rhesus Factor in Human Blood Samples using Magnetic Levitation

All procedures involving human participants have been performed in compliance with the institutional, national, and international guidelines for human ...

Take a paramagnetic gadolinium solution responsive to external magnetic fields.
Introduce fluorescently labeled Rhesus or Rh factor-positive and unlabeled Rh factor-negative blood samples.
Add anti-RhD antibody-coated low-density polymer beads and uncoated high-density beads.
Anti-RhD antibodies bind to RhD-antigens on Rh-positive RBCs, forming bead-RBC complexes.
Now, load the sample into a capillary tube and seal it. Place the tube between magnets in the magnetic levitation device.
In the magnetic field generated by like-poles-facing magnets, the paramagnetic solution exerts magnetic and buoyant forces on sample components, causing them to levitate at heights proportional to their densities.
Low-density and high-density beads levitate at the top and bottom of the capillary, respectively, establishing density height references.
Blood cells, being diamagnetic, are repelled by a strong magnetic field near the magnets and levitate at an intermediate position.
The bead-RBC complexes levitate between low-density beads and RBCs, with fluorescent cells in this layer confirming Rh-positive RBC presence.