JoVE Encyclopedia of Experiments
Encyclopedia of Experiments
This video demonstrates the detection of the Rhesus (Rh) factor in blood samples using a magnetic levitation instrument. The magnetic field aligns and levitates beads and blood cells based on density. Treating the samples with anti-Rh antibodies results in intermediate-density bead-cell complexes' formation, allowing for the differentiation of Rh-positive and Rh-negative red blood cells.
All procedures involving human participants have been performed in compliance with the institutional, national, and international guidelines for human welfare and have been reviewed by the local institutional review board.
1. Instrument setup
NOTE: Imaging levitating cells requires two rare earth neodymium magnets magnetized on the z-axis to be placed with the same pole facing each other to generate a magnetic field. The distance betw.......
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Source: Thompson, L. et al., Quantification of Cellular Densities and Antigenic Properties using Magnetic Levitation. J. Vis. Exp. (2021)
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