eoe sub articles

EoE Sub Articles

1. Evaluate the Function of TolDCs In Vitro and In Vivo
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	<li>T-cell Syngeneic Proliferation Assay
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		<li>Sterilize all the surgical instruments via autoclaving and perform the experiment in a class II biological safety cabinet with appropriate safety procedures.</li>
		<li>Prepare MACS buffer using 0.5% bovine serum albumin (BSA) and 2 mM EDTA in 500 ml PBS. Sterilize the buffer by filtering through a 0.2 &#181;m filter. 
		NOTE: Keep t...

effect of an immunomodulatory pharmacological agent on the interaction between dendritic cells and cd4+ t cells

Source: Wei, H., et al. <a href="https://app.jove.com/v/57637">Development and Functional Characterization of Murine Tolerogenic Dendritic Cells.</a> J. Vis. Exp. (135), (2018).
This video demonstrates a method of converting dendritic cells (DCs) to tolerogenic DCs (TolDCs) using an immunomodulatory pharmacological agent. This conversion is confirmed by evaluating the TolDC function through their immunosuppressive effect on CD4+ T cells.

Effect of an Immunomodulatory Pharmacological Agent on the Interaction Between Dendritic Cells and CD4+ T Cells

1. Evaluate the Function of TolDCs In Vitro and In Vivo

	T-cell Syngeneic Proliferation Assay
	
		Sterilize all the surgical instruments via autoclaving ...

Begin by adding an immunomodulatory pharmacological agent to immature dendritic cells, or DCs.
DCs express major histocompatibility complex, or MHC class-II molecule and co-stimulatory molecules, crucial for promoting T cell proliferation during immune responses.
The immunomodulatory agent engages with the DC and triggers the downregulation of MHC class-II and co-stimulatory molecules&#39; expression, thereby converting the DC into a tolerogenic dendritic cell or TolDC.
Co-culture TolDCs with na&#239;ve CD4+ T cells.
Add an antigenic peptide to the co-culture.
TolDCs internalize the peptide, subsequently processing and loading the resulting antigen onto the MHC molecule.
The antigen-bound MHC molecule translocates to the cell membrane.
Na&#239;ve CD4+ T cells interact directly with the antigen-MHC complex on TolDCs.
The interaction with the co-stimulatory molecule on the mature DC enables the full activation of the na&#239;ve T cell.
Without co-stimulation, the na&#239;ve CD4+ T cell remains dormant and cannot initiate an immune response against the presented antigen.