a mouse tail snip procedure for peripheral blood collection and plasma isolation

A Mouse Tail Snip Procedure for Peripheral Blood Collection and Plasma Isolation

To collect a peripheral blood sample, first, gently position the mouse in a disinfected small animal restrainer, and secure the tube to minimize the animal&#39;s movements.
Next, scrub the tail with fresh isopropyl alcohol, followed by the application of topical anesthetic cream.
Using very sharp surgical scissors, snip about 0.5 to 1 millimeter from the tip of the tail, and collect approximately 80 microliters of peripheral blood into heparinized capillary tubes.
Use a bulb syringe to transfer the blood samples into labeled potassium-EDTA microtainer tube, and invert the tube 20 times to prevent coagulation.
After centrifuging blood collection tubes per manufacturer&#39;s instructions, carefully collect the plasma layer and store the aliquots at minus 20 degrees Celsius, and aliquot the plasma for minus 20 degrees Celsius storage.