JoVE Encyclopedia of Experiments
Encyclopedia of Experiments
This video demonstrates a technique to visualize exocytosis in mast cells. The cells are incubated with FITC-dextran — a fluorescent reporter — which is taken up by cells inside secretory granules (SGs), where the acidic pH quenches its fluorescence. Upon stimulation of granule exocytosis, the medium's higher pH induces reporter fluorescence inside the fused SGs, which is visualized using a fluorescence microscope.
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Source: Klein, O., et al. Imaging FITC-dextran as a Reporter for Regulated Exocytosis J. Vis. Exp. (2018).
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