JoVE Encyclopedia of Experiments
Encyclopedia of Experiments
This video demonstrates a method to quantify fungal infection in zebrafish larvae. Fungal spores are microinjected into the larval hindbrain ventricle, and the larvae are incubated to allow infection development. Infected larvae are harvested at defined intervals, and infection progress is assessed by enumerating colony-forming units.
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Preparation of Aspergillus spores for injection
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Source: Thrikawala, S., et al. Infection of Zebrafish Larvae with Aspergillus Spores for Analysis of Host-Pathogen Interactions. J. Vis. Exp. (2020).
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