Synthesis of Phase-shift Nanoemulsions with Narrow Size Distributions for Acoustic Droplet Vaporization and Bubble-enhanced Ultrasound-mediated Ablation

Summary

Phase-shift nanoemulsions (PSNE) can be vaporized using high intensity focused ultrasound to enhance localized heating and improve thermal ablation in tumors. In this report, the preparation of stable PSNE with a narrow size distribution is described. Furthermore, the impact of vaporized PSNE on ultrasound-mediated ablation is demonstrated in tissue-mimicking phantoms.

Abstract

High-intensity focused ultrasound (HIFU) is used clinically to thermally ablate tumors. To enhance localized heating and improve thermal ablation in tumors, lipid-coated perfluorocarbon droplets have been developed which can be vaporized by HIFU. The vasculature in many tumors is abnormally leaky due to their rapid growth, and nanoparticles are able to penetrate the fenestrations and passively accumulate within tumors. Thus, controlling the size of the droplets can result in better accumulation within tumors. In this report, the preparation of stable droplets in a phase-shift nanoemulsion (PSNE) with a narrow size distribution is described. PSNE were synthesized by sonicating a lipid solution in the presence of liquid perfluorocarbon. A narrow size distribution was obtained by extruding the PSNE multiple times using filters with pore sizes of 100 or 200 nm. The size distribution was measured over a 7-day period using dynamic light scattering. Polyacrylamide hydrogels containing PSNE were prepared for in vitro experiments. PSNE droplets in the hydrogels were vaporized with ultrasound and the resulting bubbles enhanced localized heating. Vaporized PSNE enables more rapid heating and also reduces the ultrasound intensity needed for thermal ablation. Thus, PSNE is expected to enhance thermal ablation in tumors, potentially improving therapeutic outcomes of HIFU-mediated thermal ablation treatments.

Protocol

1. Preparation of Phase-shift Nanoemulsion (PSNE)

1. Dissolve 11 mg DPPC and 1.68 mg DSPE-PEG2000 in chloroform
2. Evaporate the organic solvent to form a dry lipid film in a glass round-bottom flask
3. Dessicate the lipid film overnight
4. Rehydrate the lipid film with 5.5 ml of phosphate-buffered saline (PBS)
5. Heat solution in a 45 °C water bath until lipid film dissolves, vortexing periodically
6. Transfer lipid solution into 7 ml vial
7. Sonicate lipid solution for 2 min at 20% amplitude
8. Divide solution into two vials of 2.5 ml each (discard remaining 0.5 ml)
9. Add 2.5 ml PBS to each vial
10. Place each vial in a 0 °C ice-water bath
11. Add 50 μl DDFP to each vial
12. Sonicate each vial in the ice-water bath using the following settings: 25% amplitude, pulsed mode (10 sec on, 50 sec off), 60 sec total on time
13. Transfer PSNE solutions to 20 ml scintillation vials
14. Add 5 ml PBS to each vial, resulting in 10 ml final volume
15. Assemble extruder following directions provided by manufacturer
    1. Rinse each part with deionized water
    2. Place the stainless steel support disc in the center of the filter support base
    3. Place the stainless steel mesh on top of the stainless steel support disc
    4. Using tweezers, place an extruder drain disc membrane (shiny side up) on the stainless steel mesh
    5. Using tweezers, place the extruder filter (shiny side up) on the drain disc membrane
    6. Carefully place the small O-ring on the filter and place the thermobarrel and extruder top above the support base
    7. Partially tighten each wing-nut first, then completely tighten the wing-nuts by hand in an alternating fashion
    8. Connect the extruder to a nitrogen gas line
    9. To prime the extruder, pipette 10 ml deionized water into the top sample port, cap the opening, and tighten the vent valve
    10. Slowly open the nitrogen gas line to increase the pressure, forcing the sample through the membranes, and collect the sample from the outlet tubing
    11. After use, disassemble in reverse order, rinse the extruder parts with deionized water, and discard the membrane filter and membrane drain disc
16. For 100 nm droplets only, pre-condition PSNE by extruding 10 times through 200 nm filter
17. Extrude PSNE 16 times through 100 nm or 200 nm filter to obtain narrow size distribution

2. Preparation of Polyacrylamide Hydrogel Containing PSNE

1. Prepare 24% BSA solution by diluting 1.2 g BSA powder in 5 ml deionized water
2. Prepare 10% APS solution by diluted 0.1 g APS powder in 1 ml deionized water
3. In the following order, mix 2.1 ml acrylamide solution, 1.2 ml Tris buffer, 0.1 ml 10% APS, 4.5 ml 24% BSA solution, and 3.6 ml deionized water in plastic chamber
4. Heat to 40 °C and place under vacuum for 1 hr
5. Add 480 μl of PSNE and thoroughly mix by gently swirling the plastic chamber.
6. Add 12 μl TEMED and place the chamber in a 12 °C water bath for 2 hr

3. Representative Results

A schematic of the setup for ultrasound experiments with tissue-mimicking hydrogel phantoms is shown in Figure 1. This protocol results in lipid-coated perfluorocarbon droplets with a narrow size distribution that are stable in solution for at least a week. The size distribution measured with dynamic light scattering (90Plus Particle Size Analyzer, Brookhaven Instruments, Holtsville, NY) is shown in Figure 2 for PSNE extruded using 100 and 200 nm filters. The PSNE effective diameter over time, measured using dynamic light scattering, is listed in Table 1, demonstrating that PSNE are stable for at least a week. B-mode images of PSNE before and after vaporization in a polyacrylamide hydrogel are shown in Figure 3. Also, a lesion formed by 15 sec of HIFU-mediated heating in a polyacrylamide hydrogel containing albumin and PSNE is shown in Figure 4. The asymmetric shape of the lesion is a result of prefocal heating that occurs due to the presence of the bubble cloud in the ultrasound path. It is important to note that prefocal heating and lesion formation due to scatter from bubbles can be minimized by reducing the transmitted acoustic power.

Figure 1. Schematic diagram of experimental setup for ultrasound experiments with tissue-mimicking hydrogels.

Figure 2. Size distribution of PSNE extruded through 100 nm or 200 nm filters, measured using dynamic light scattering. The units of the ordinate axes are based on the intensity of scattered light from particles of a certain size relative to the total scattered light intensity from the sample.

Figure 3. B-mode images (a) before and (b) after PSNE vaporization in a polyacrylamide hydrogel. The arrow indicates the focal region where a bubble cloud was formed by PSNE vaporization.

Figure 4. Images of polyacrylamide hydrogel containing albumin and PSNE (a) before and (b) after vaporization and sonication with HIFU, demonstrating lesion formation as a result of ultrasound-induced heating. The ultrasound center frequency was 3.3 MHz. The ultrasound signal consisted of an initial 30-cycle, 6.4 W pulse to vaporize PSNE, immediately followed by 15 sec of continuous ultrasound at 0.77 W.

Days after extrusion	Extruded with 200 nm filter		Extruded with 100 nm filter
	Mean Dia. (nm)	Std. Dev. (nm)	Mean Dia. (nm)	Std. Dev. (nm)
1	182.9	4.9	118.0	0.9
7	177.7	2.5	124.8	3.1

Table 1. Mean diameter and standard deviation of PSNE at one and seven days after extrusion with 100 nm and 200 nm filters.

Discussion

High-intensity focused ultrasound (HIFU) is used clinically to thermally ablate tumors.¹ To enhance localized heating and improve thermal ablation in tumors, lipid-coated perfluorocarbon droplets have been developed which can be vaporized by HIFU. The vasculature in many tumors is abnormally leaky due to their rapid growth.² Thus, nanoparticles are able to penetrate the fenestrations and passively accumulate within tumors, a process known as the enhanced permeability and retention (EPR) effect...

Materials

Name	Company	Catalog Number	Comments
DPPC	Avanti Lipids, Alabaster, AL, USA	850355P	1,2-dipalmitoyl-sn-glycero-3-phosphocholine
DSPE-PEG2000	Avanti Lipids, Alabaster, AL, USA	880120P	1,2-distearoyl-sn-glycero-3-phosph–thanolamine-N-[methoxy(polyethylene glycol)-2000] (ammonium salt)
DDFP	Fluoromed, Round Rock, TX, USA	CAS: 138495-42-8	Dodecafluoropentane (C5F12)
PBS	Sigma-Aldrich, St. Louis, MO, USA	P2194	Phosphate-buffered saline
Chloroform	Sigma-Aldrich, St. Louis, MO, USA	372978	Chloroform
Acrylamide	Sigma-Aldrich, St. Louis, MO, USA	A9926	40% 19:1 acrylamide/bis-acrylamide
Tris buffer	Sigma-Aldrich, St. Louis, MO, USA	T2694	1M, pH 8, trizma hydrochloride and trizma base
BSA	Sigma-Aldrich, St. Louis, MO, USA	A3059	Bovine serum albumin
APS	Sigma-Aldrich, St. Louis, MO, USA	A3678	Ammonium persulfate solution
TEMED	Sigma-Aldrich, St. Louis, MO, USA	87689	N,N,N',N'-Tetramethylethylenediamine
Equipment
Sonicator (3 mm tip)	Sonics Materials, Inc., Newtown, CT, USA	Vibra-Cell
Water bath	Thermo Fisher Scientific, Waltham, MA, USA	Neslab EX-7
Extruder	Northern Lipids, Burnaby, BC, Canada	LIPEX
Extruder Filters	Whatman, Piscataway, NJ, USA	Nuclepore #110605 and #110606
Extruder Drain Disc	Sterlitech Corporation, Kent, WA, USA	#PETEDD25100
Plastic chamber	U.S. Plastic Corporation, Lima, OH, USA	#55288, 1 3/16"x1 3/16"x2 7/16"