Synergetic Use of Neural Precursor Cells and Self-assembling Peptides in Experimental Cervical Spinal Cord Injury

Summary

Treating cervical spinal cord injury with both self-assembling peptides (SAP) and neural precursor cells (NPC), together with growth factors, is a promising approach to promote regeneration and recovery. A contusion/compression aneurysm clip rat model of cervical SCI and combined treatment involving SAP injection and NPC transplantation is established.

Abstract

Spinal cord injuries (SCI) cause serious neurological impairment and psychological, economic, and social consequences for patients and their families. Clinically, more than 50% of SCI affect the cervical spine¹. As a consequence of the primary injury, a cascade of secondary mechanisms including inflammation, apoptosis, and demyelination occur finally leading to tissue scarring and development of intramedullary cavities^2,3. Both represent physical and chemical barriers to cell transplantation, integration, and regeneration. Therefore, shaping the inhibitory environment and bridging cavities to create a supportive milieu for cell transplantation and regeneration is a promising therapeutic target⁴. Here, a contusion/compression model of cervical SCI using an aneurysm clip is described. This model is more clinically relevant than other experimental models, since complete transection or ruptures of the cord are rare. Also in comparison to the weight drop model, which in particular damage the dorsum columns, circumferential compression of the spinal cord appears advantageous. Clip closing force and duration can be adjusted to achieve different injury severity. A ring spring facilitates precise calibration and constancy of clip force. Under physiological conditions, synthetic self-assembling peptides (SAP) self-assemble into nanofibers and thus, are appealing for application in SCI⁵. They can be injected directly into the lesion minimizing damage to the cord. SAPs are biocompatible structures erecting scaffolds to bridge intramedullary cavities and thus, equip the damaged cord for regenerative treatments. K2(QL)6K2 (QL6) is a novel SAP introduced by Dong et al.⁶ In comparison to other peptides, QL6 self-assembles into β-sheets at neutral pH⁶.14 days after SCI, after the acute stage, SAPs are injected into the center of the lesion and neural precursor cells (NPC) are injected into adjacent dorsal columns. In order to support cell survival, transplantation is combined with continuous subdural administration of growth factors by osmotic micro pumps for 7 days.

Introduction

More than 50% of spinal cord injuries are related to the cervical spine. In the clinical setting two major pathophysiological mechanisms are described: the initial contusion of the spinal cord and subsequently, the ongoing compression caused by bone fractures, hemorrhages or tissue swelling.

The aneurysm clip contusion/compression model mimics both pathophysiological mechanisms: snapping the clip produces a contusion and the duration of clipping represents the compression component, conceding that the compression in clinical settings caused by bone fractures, hemorrhages or tissue swelling last significant longer. The used aneurysm clip is modified by a ring spring guaranteeing exact and reproducible clipping force. Especially in comparison to the hemi-transection or the contusion model, this aneurysm clip model mimics best clinical settings. While patients with thoracic injuries suffer from paraplegia, most patients with cervical injuries are tetraplegic and completely dependent. The anatomical structure of the cervical cord, however, shows significant differences compared to the thoracic or lumbar spine, and thus, is addressed in particular in this protocol.

The development of intramedullary cavities and tissue scarring are obstacles for recovery and regeneration. To overcome these barriers the use of scaffold material is a promising approach. Self-assembling peptides can be injected directly into the epicenter of the lesion. There they assemble into nano-fiber scaffolds bridging the cavity and improve the inhibitory environment by reducing inflammation and tissue scaring. While rigid materials cause considerable damage of the spinal cord during implantation, the fluid peptides can be injected safely and without severe additional damage.

Improving the inhibitory environment with self-assembling peptides before stem cell transplantation, hence, support cell integration, differentiation and finally, functional recovery, after cervical spinal cord injury.

Protocol

NOTE: The following experimental protocol was approved by the animal care committee of the University Health Network (Toronto, Canada) and is in accordance with the policies established in the guide to the care and use of experimental animals prepared by the Canadian council of animal care.

1. Cervical Aneurysm Clip Contusion/Compression Model

1. Before surgery autoclave instruments and keep sterile conditions during the entire surgcial procedure by putting the instruments in a 70% alcohol bath.
2. Anaesthetize Wistar rats (250-270 g) with a combination of oxygen (O₂), nitrous oxide (N₂O) (1:1), and 1.8-2.2% Isoflurane and support spontaneous breathing via a gas anesthesia mask. For induction of anesthesia start with 5% Isoflurane for 1 minute and reduce afterwards. Before starting surgery, control depth of anesthesia by giving a painful stimulus (e.g. at the paws). Apply fatty ointments in the eyes to avoid dryness and to prevent subsequent infections.
3. Put the rats onto a heating cushion (37 °C) and fix the head into a stereotactic frame.
4. Shave the surgical region around the cervical spine and disinfect with povidone iodine and 70% alcohol.
5. Make a midline incision above the spine from the cervical vertebra (C2) reaching to the prominent processus of the thoracic vertebral body 2 (T2).
6. Cut through the outer layer of the vertebral muscles directly on the midline (to avoid bleeding) in a cranio-caudal direction and further dissect the deeper muscle layers bluntly until you reach the processus spinosus and the laminae. Insert retractors.
7. Orientate the prominent processus spinosus of the vertebra body T2 to observe the targeted levels for laminectomy. After identification and micro-surgical preparation of the selected laminae, cut through the ligamenti flavae to loosen the laminae and the processus spinosus. Finally, cut through the laminae with a bone clipper lateral to the spinal cord and remove those gently, avoiding any compression of the spinal cord itself.
   NOTE: Most common levels are C5/6, C6/7, or C7/T1. The perioperative mortality rate increases the more rostral the level of injury. Bleeding from the paravertebral venous sinus is common and can be addressed by careful compression with sponge.
8. Before inserting the clip to traumatize the cord, identify emerging nerve roots to spare them from clipping (especially at level C5/6).
9. In order to ensure smooth clip induction, loosen the ventral dura from the dorsal side of the vertebral bodies with a hook and prepare a corridor for the clip.
10. Finally, insert the open clip and let it snap shut (rapid closure) to achieve a contusion injury. Clip closing force and duration of clip closure determine the intensity of the trauma and the extent of compression. Commonly used are clip forces of between 15-35 g and a clipping duration of e.g. 1 min. (Figure 1).
11. After removal of the clip, adapt muscles in 2 layers and close the wound.
12. Stop anesthesia and let the animal wake up under your continuous observation until it regains sufficient consciousness for sternal recumbency. Finally, put the rat in a single cage and follow post-operative treatment guidelines.
13. Since animals struggle with the severity of this type of injury, you must pay special attention to post-operative treatments:
    1. Administer painkillers (Buprenorphine and Meloxicam for 3 days and 5 days, respectively and according to the clinical symptoms).
    2. Give additional saline solution subcutaneously for 3 days (2 times a day, 5-10 milliliter (ml)).
    3. Provide antibiotics in the drinking water 2 days prior and until 7 days post surgery (e.g. Moxifloxacine)
    4. Squeeze the urinary bladder 2-3 times a day until recovery of bladder function is constantly apparent.
    5. Observe neurological deficits and physiological condition of the operated animals at least once a day.

2. Injecting SAPs and NPCs (14 days after injury)

1. Induce anesthesia as described in 1.1. to 1.3, fix the head of the rat in a stereotactic frame, remove the stitches or wound clips, and disinfect the wound and the surgical area with povidone iodine and 70% alcohol.
2. Carefully dissect the paravertebral muscles, insert retractors, remove scar tissue microscopically from the dura, and re-expose the lesion site.
3. Prepare SAPs in a concentration of 1% (w/v) to perform an extracellular matrix gel. QL6 SAPs have a physiologically compatible pH and do not need to be buffered prior to injection. For visualization of SAPs in the spinal cord, use a fluorescent derivate of QL6 (QL6-FITC).
4. Inject SAPs (5 microliter (μl) into the center of the lesion, distributed in 2 portions, each 2.5 μl bilateral of the midline. Use a Hamilton syringe connected to the stereotactic frame with a micro glass capillary (100 micrometer (μm) outer diameter (OD)). Open the dura carefully with the tip of a sharp needle, and insert the glass capillary stereotactically 2 millimeter (mm) into the traumatized spinal cord.
5. After injecting 1/3 of the volume, remove the needle to 1.5 mm depth, and after a further 1/3 to 1 mm. After injection of the entire volume, and before removal of the syringe, wait 5 minutes to stabilize gel formation.
6. To generate NPCs, use adult DsRed mice (or YFP positive mice, green) and isolate and cultivate them from the paraventrical zone^4,18,21.
7. Assess viability of NPCs by Trypan Blue staining indicating the presence of ~90% live cells in the cell suspension. Dilute the cells in growth medium (50 x 103 live cells/μl) and then use them for cell transplantation.
8. Make four 2 μl (8 μl total volume, containing 4 x 10⁵ NPCs) intraspinal injections bilaterally at 2 mm rostral and caudal of the injury site. After opening of the dura, insert the Hamilton micro glass capillary 1.5 mm below the dorsal surface of the spinal cord and inject 2 μl of the cell suspension. Choose an injection rate at about 0.5 μl /min (min).
9. At the end of each injection and before removal of the capillary out of the cord, wait for at least 1 min allowing tissue stretching to accommodate the new cell volume. (Figure 2)

3. Implantation of Subdural Pumps for Growth Factor Application

1. In order to enrich the cerebral spinal fluid (CSF) with growth factors supporting cell survival, use micro-osmotic pumps diluting growth factors sub-durally throughout 7-14 days, with a dilution rate of 0.5 μl/hr, a catheter diameter of 0.04 cm OD, and a reservoir volume of 100 μl.
2. Choose preferred growth factors (e.g., brain derived growth factor (BDGF), epidermal growth factor (EGF), fibroblast growth factor (FGF)), fill the pumps 6-10 hr prior implantation, and equilibrate pumps in a water bath at 37 °C.
3. Immediately after NPC injections, prepare a subcutaneous recess to place the pump. Preferred locations are the lateral flanks of thoraco- abdominal region avoiding major local discomfort caused by the pump itself.
4. To gain the highest concentration of growth factors, ensure that the open tip of the catheter ends close to the lesion site. Therefore, perform a skip-laminectomy of the adjacent upper or lower level. For example, if the SCI is at C7/T1, perform a small laminectomy of C5.
5. Put the pump into the subcutaneous recess, shorten the catheter to the necessary length, and secure it with several sutures (6.0) on the paravertebral muscles avoiding any movement-associated dislocation.
6. After opening the dura (e.g., at C5) with the sharp tip of a needle, introduce the catheter into the subdural space and let it glide in a caudal direction without any resistance and without injuring the cord. The skipped lamina of e.g. C6 serves as additional point of fixation and stabilization of the catheter.
7. Make sure that the catheter runs smoothly and does not fold.
8. Close muscles by layer, and skin, with sutures or clips. (Figure 3)
9. Stop anesthesia and let the animal wake up under your continuous observation until it regains sufficient consciousness for sternal recumbency. Finally, put them back in a single cage and follow post-operative treatment guidelines.
10. Provide special postoperative treatment, even though, animals might have recovered partly at this time-point:
    1. Administer painkillers (Buprenorthine and Meloxicam for 3 days and 5 days, respectively and according to the clinical symptoms).
    2. Provide antibiotics in water bottles 2 days prior until 7 days post-surgery (e.g. Moxifloxacine).
    3. Continue squeezing the urinary bladder, if still necessary.
    4. Give additional subcutaneous fluids, if the rats appear dehydrated.
    5. Keep observing the neurological function and physiological condition of the operated animals at least once a day.
    6. Administer immunosuppression treatment 2 days prior NPC injection and until 7 days post transplanation (minocycline) and until sacrifice (sandimmune), respectively.

4. Tissue Assessment

1. Sacrifice animals at the end of the observation time (e.g. 4 weeks after SCI) in deep anesthesia (5% Isoflurane for 2-3 min) and perfuse them transcardially with 50 ml cold (4 °C) saline followed by 150 ml cold 4% paraformaldehyde in 0.1 M phosphate-buffered saline (PBS).
2. Remove the spinal cord and put it in 4% paraformaldehyde in 0.1 M phosphate-buffered saline (PBS) for 24 hr.
3. Put the spinal cords in 25% sucrose solution for cryoprotection for 48 hr.
4. Provide longitudinal cryosections with a thickness of <30 μm.
5. For immunohistochemical staining of all cell-nuclei providing a background use DAPI (1:1,000). DsRed positive NPCs appear red, QL-6 FITC appears green, and both thus need not to be stained specially. (Figure 4).
6. For scanning electron microscopy (SEM) let samples soak in glutaraldehyde at 4 °C for 2 hr, dehydrate them slowly in 10% increment steps of ethanol for 5 min and place them in a pressurized liquid CO₂ siphon for 1 hr. Coat scaffolds with gold using a sputter coater. Take images with a Hitachi S-3400N scanning electron microscope. (Figure 5)

Results

When performing the above-described procedure, you will get a SAP scaffold bridging the cavity and offering an improvement of the inhibitory environment, less tissue scaring and an increase in NPC survival. Figure 4 shows a longitudinal section of a rat spinal cord obtained at the injury site 6 weeks after SCI and 4 weeks after QL6 SAP injection and NPC transplantation. QL6 peptides were successfully injected into the cord, aggregated in the epicenter and diffused rostro-caudally in the penumbra. Electro...

Discussion

This protocol has been developed to enable the reader to perform a cervical injury model in rats and to use a combined treatment approach with SAPs and NPCs promoting better recovery after cervical SCI.

Particularly in comparison to other cervical trauma models, such as the (hemi)-transection model or the weight drop and concussion models, the clip contusion/compression model represents both major pathophysiological trauma mechanisms – contusion and compression- and therefore mimics best...

Materials

Name	Company	Catalog Number	Comments
Aneurysmal clip	SharpTech
Surgical microscope	Leica
Micro injection system	World Precision Instruments, Inc.
Small animal stereotaxic instrument	David Kopf Instruments
Hamilton syringe	Hamilton company
Subdural pumps	Alzet osmotic micro pump 1007D
Surgical instrument	Fine Science tools
Isoflurane USP	Pharmaceutical Partners of Canada Inc.
0.9% Sodium Chloride injection USP	Baxter
7.5% Povidone iodine	Purdue Pharma
70% Isopropyl alcohol USP	GreenField Ethanol Inc.
QL6 SAP	Covidien
0.4% Trypan blue	Gibco
Platelet-Derived Growth Factor (PDGF)	Sigma
Epidermal Growth Factor (EGF)	Sigma
Fibroblast Growth Factor (FGF)	Sigma