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In This Article

  • Summary
  • Abstract
  • Introduction
  • Protocol
  • Results
  • Discussion
  • Disclosures
  • Acknowledgements
  • Materials
  • References
  • Reprints and Permissions

Summary

Here, we present a robust, fast and scalable cardiomyocyte differentiation protocol for human pluripotent stem cells (hPSCs). Cardiomyocytes derived using this large-scale method can provide sufficient cell numbers for their effective use in human cardiovascular disease modeling, high-throughput drug screening, and potentially clinical applications.

Abstract

Maximizing the benefit of human pluripotent stem cells (hPSCs) for research, disease modeling, pharmaceutical and clinical applications requires robust methods for the large-scale production of functional cell types, including cardiomyocytes. Here we demonstrate that the temporal manipulation of WNT, TGF-β, and SHH signaling pathways leads to highly efficient cardiomyocyte differentiation of single-cell passaged hPSC lines in both static suspension and stirred suspension bioreactor systems. Employing this strategy resulted in ~ 100% beating spheroids, consistently containing > 80% cardiac troponin T-positive cells after 15 days of culture, validated in multiple hPSC lines. We also report on a variation of this protocol for use with cell lines not currently adapted to single-cell passaging, the success of which has been verified in 42 hPSC lines. Cardiomyocytes generated using these protocols express lineage-specific markers and show expected electrophysiological functionalities. Our protocol presents a simple, efficient and robust platform for the large-scale production of human cardiomyocytes.

Introduction

Human pluripotent stem cells (hPSCs), including human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs), have the ability of self-renewal and the capacity to differentiate into cells of the three embryonic germ layers 1,2. Due to these characteristics, hPSCs provide a valuable and unlimited source for the generation and scalable production of disease-relevant cell types for modeling human disease 3-5, for high-throughput drug screening and toxicity assays 6,7 and potentially for clinical applications 8. Generation of cardiomyocytes from hPSCs provides the opportunity to specifically investigate the mechanisms of complex human cardiovascular diseases and their possible treatments, previously beyond the scope of our capabilities due to the lack of relevant animal models and/or the availability of affected primary tissues.

All of the aforementioned applications of hPSCs necessitate the production of massive numbers of highly enriched and functional cardiomyocytes. Thus, the availability of an efficient, reproducible and scalable in vitro cardiac differentiation protocol suitable for multiple hPSC lines is crucial. Conventional cardiomyocyte differentiation protocols have employed different strategies such as embryoid body formation 9, co-culture techniques 10, induction with cocktails of cytokines 11 and protein transduction methods 12. In spite of advances in these techniques, most still suffer from poor efficiency, require expensive growth factors, or offer limited universality when attempting to use multiple hPSC lines. To date, these challenges have set limits to the production of hPSC-derived cardiomyocytes for cell therapy studies in animal models, as well as in the pharmaceutical industry for drug discovery 13. Therefore, the development of robust and affordable techniques for large-scale production of functional hPSC-derived cardiomyocytes in scalable culture systems would largely facilitate their commercial and clinical applications.

In this manuscript, we report the development of a cost-effective and integrated cardiac differentiation system with high efficacy, reproducibility and applicability to hESCs and hiPSCs generated from a variety of sources and culture methods, including a method for the large-scale production of highly enriched populations of hPSC-derived cardiomyocytes using a bioreactor. Additionally, we have optimized this protocol for hPSC lines not adapted to feeder free and/or single cell culture, such as newly established hiPSCs or large cohorts of hPSC lines relevant to analysis of disease mechanism.

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Protocol

1. Preparation of Culture Media, Coating of Cell Culture Plates and Maintenance of Undifferentiated hPSCs

  1. Media Preparation
    Note: Sterilize media using a 0.22 µm filtration device and store at 4 °C protected from light for up to 4 weeks. Reagent names, suppliers and catalog numbers are listed in Materials Table.
    1. For Mouse Embryonic Fibroblasts (MEF) Medium, combine 445 ml DMEM, 50 ml Fetal Bovine Serum (FBS) and 5 ml cell culture media (e.g., Glutamax).
    2. For hESC Medium, combine 390 ml Knockout-DMEM (KO-DMEM), 100 ml Knockout Serum Replacement (KO-SR), 5 ml cell culture media, 5 ml MEM minimum essential amino acids solution and 0.5 ml 55 mM β-mercaptoethanol.
      CAUTION: β-Mercaptoethanol is toxic. Avoid inhalation, ingestion and skin contact.
    3. For RPMI-B27 (RB) Medium, combine 475 ml RPMI 1640, 10 ml B27 minus insulin, 5 ml cell culture media, 5 ml MEM minimum essential amino acids solution, 5 ml penicillin/streptomycin and 0.5 ml 55 mM β-mercaptoethanol.
    4. For the Dissociation Solution (DS), combine 10 ml 0.05% trypsin, 4 ml KO-SR, 1 ml collagenase type IV (1 mg/ml), 5 ml KO-DMEM and 20 µl CaCl2(1 M).
    5. For Feeder-cell Conditioned Medium, add 15 ml hESC medium (without bFGF) to a T75 flask with confluent feeder cells (MEF or human foreskin fibroblasts) which have been previously inactivated by treatment with either mitomycin C or by irradiation. Harvest conditioned medium after 24 hr and replace with fresh hESC medium. Cells can be used for up to 2 weeks.
  2. Coating of Cell Culture Plates
    1. ECM Gel Coating:
      1. Upon purchase, thaw the ECM gel extract at 4 °C until it is in an evenly consistent liquid state, as per the manufacturer's instructions. Dilute at a ratio of 1:2 in cold KO-DMEM medium, aliquot and store at -20 °C.
        Note: During the preparation of the ECM gel, keep all materials required for use in the aliquotting and coating procedure cold. The concentration of ECM gel varies with batch number. Ensure the concentration is noted on aliquots. Aliquots can be kept at -20 °C for up to 6 months.
      2. Thaw an ECM gel aliquot at 4 °C. When thawed, add cold KO-DMEM medium for a final concentration of 0.34 mg/ml. Pipette well and add 0.75 ml per one well of a 6-well plate. Incubate for 1 hr at 37 °C.
    2. Mitotically Inactivated Mouse Embryonic Fibroblast Feeder Cell (MEF) Coating
      Note: Preparation of MEF feeder cells has been described previously. 14
      1. Coat a 6-well cell culture plate with Attachment Factor (AF) or 0.1% gelatin (see step 1.2.3) at RT for 5 min (0.75 ml per one well of a 6-well plate). Remove and leave plate in the biosafety cabinet (BSC) to dry.
      2. Thaw MEF by transferring a frozen vial to a 37 °C water bath for approximately 2 - 3 min.
      3. Add 7 ml MEF medium to a 15 ml tube. When the vial of cells is thawed, slowly transfer the contents to the 15 ml tube. Centrifuge at 300 x g for 4 min. Aspirate the supernatant and resuspend the cells in MEF medium.
      4. Count the cells and plate 1 × 106cells per 6 well plate (~ 1.7 x 105 cells/well). Transfer to a 37 °C / 5% CO2 incubator and allow cells to attach O/N before use.
    3. Gelatin Coating:
      1. Dissolve 1 g of gelatin powder in ultrapure water to make a 0.1% (w/v) solution. Incubate for 15 min at 37 °C then autoclave the solution at 121 °C for 15 min. When cooled, add to the required number of wells (0.75 ml per one well of a 6-well plate) and incubate for 15 min at 37 °C.
        Note: Any remaining 0.1% gelatin solution can be kept at 4 °C for up to 1 month.
    4. Laminin Coating:
      1. Thaw laminin (1 mg/ml) at 4 °C until thawed. To 5 µl of laminin solution, add 1 ml of cold PBS. Pipette well then add to the required number of wells (0.75 ml per one well of a 6-well plate) and incubate for 1 hr at 37 °C.
    5. Silicon Coating of Spinner Flask Internal Surface:
      1. Thoroughly wash a 100 ml spinner flask (with 1 glass pendulum) with distilled water, using a cleaning brush to remove any dust and/or culture residue. Fill with 70% ethanol and after 30 min rinse with distilled water. Add 5 M NaOH and leave O/N.
      2. Remove NaOH solution and rinse the flask under running water for 5 min. Fill the flask with 1 M HCl and leave for 15 min. Wash the flask with running water for 5 min, then twice with double distilled water to completely remove any trace of HCl. Leave the flask to dry completely in the BSC.
      3. Add 1.5 ml of siliconizing solution to the flask and rotate horizontally to cover all surfaces. Repeat the same procedure for the glass pendulum.
      4. Heat the coated flask in a dry oven at 100 °C for 1 hr or allow to dry O/N at RT. If heated in an oven, leave to cool to RT for 30 - 60 min.
      5. Rinse the flask 3 times with deionized water for 15 min each, then sterilize by autoclaving at 121 ˚C for 20 min.
  3. Maintenance of Undifferentiated hPSCs
    Note:     For each of the protocols described, unless specifically stated, cells are grown and cultured in wells of 6-well culture plates and volumes will be given appropriate for this format.
    1. hPSCs Cultured as Undifferentiated Spheroids in a Static Suspension System
      Note: Cells used in these steps should already be adapted to single-cell culture. 15
      1. Start experiment with hPSCs cultured on ECM gel in a 6-well culture plate at approximately 70 - 80% confluency. Remove any differentiated colonies using the stereomicroscope in the BSC. Add 10 µM ROCK inhibitor Y-27632 and incubate at 37 °C for 1 hr.
        Note: For removing the differentiated colonies, use a pipette tip to detach and gently remove all the differentiated parts manually under the stereomicroscope. Be careful not to remove undifferentiated colonies.
      2. Remove cells from incubator and aspirate medium. Wash the cells with 1 ml PBS, remove and add 0.5 ml cell dissociation enzyme. Incubate the cells for 4 - 5 min at 37 °C.
      3. Add 1 ml hESC medium and harvest the cells using a cell scraper. Dissociate the cells into single cells using a p1,000 pipette. Count the viable cells using Trypan blue and a hemocytometer.
      4. Resuspend cells to 2 × 105 viable cells/ml in feeder-cell conditioned medium supplemented with 100 ng/ml bFGF and 10 µM ROCK inhibitor Y-27632. Transfer cells to ultra-low attachment plates using a 5 ml pipette (3 ml per well of 6-well plate).
      5. After 2 days, carefully remove cells from incubator and aspirate half of the medium (approximately 1.5 ml). Replace with fresh conditioned medium supplemented with 100 ng/ml bFGF .
      6. Change half of the medium every day with conditioned medium supplemented with 100 ng/ml bFGF until day 5. Now, either further culture cells, passage for continued undifferentiated culture (step 1.3.1.2 - 5), or use for cardiac differentiation (Section 2). If continuing culture, passage cells every 4 - 8 days.
    2. hPSCs Cultured on Feeder Cells and Passaged Using Collagenase Type IV
      1. Before passaging hPSCs, remove any differentiated colonies using the stereomicroscope in the BSC. Aspirate the medium and wash cells with 1 ml PBS per well. Remove then add 0.75 ml collagenase type IV (1 mg/ml) and incubate at 37 °C for 5 - 15 min, as required to have the edges of the colonies starting to peel off.
      2. Aspirate collagenase and add 1 ml of hESC medium per well. Detach colonies to small clusters using a cell scraper, then transfer the cells using a p1,000 pipette into a 15 ml tube. Be careful not to break up the clusters into small pieces.
      3. Wash the well with 1 ml hESC medium to collect any remaining cells and add to 15 ml tube. Centrifuge at 100 x g for 2 min. Aspirate the supernatant and resuspend cells in hESC medium supplemented with 100 ng/ml bFGF.
      4. Remove a MEF coated plate from the incubator and aspirate the MEF medium. Transfer the cells at a ratio between 1:3 and 1:10, as appropriate. Change medium daily with fresh hESC medium supplemented with 100 ng/ml bFGF. Passage cells every 4 - 8 days.

2. Differentiation of hPSCs as Spheroids in a Static Suspension System

  1. Start experiment using day 5 undifferentiated spheroids as prepared in Section 1.3.1.
    Note: At this stage, the average size of spheroids should be 175 ± 25 µm. A minimum of 50 spheroids should be used when starting a differentiation experiment.
  2. Transfer spheroids into a 15 ml tube using a 5 ml pipette. Wash the well with 1 ml RB medium to collect any remaining spheroids and add to the same 15 ml tube. Leave the cells for 5 min until spheroids have sedimented to form a loose pellet (DO NOT centrifuge). Aspirate the supernatant, being careful not to take any spheroids.
  3. Add 3 ml RB medium supplemented with 12 µM CHIR99021. Transfer the cells back into one well of a 6-well ultra-low attachment plate. After 24 hr, repeat step 2.2.
    Note: RB medium is light sensitive. When working with RB medium, keep the BSC light switched off.
  4. Add 3 ml RB medium to the cell pellet. Transfer the cells back into one well of a 6-well ultra-low attachment plate. After 24 hr, repeat step 2.2.
  5. Add 3 ml RB medium supplemented with IWP2, purmorphamine and SB431542 (5 µM each). Transfer the cells back into one well of a 6-well ultra-low attachment plate. After 2 days repeat step 2.2.
  6. Resuspend the cells in 3 ml of RB medium and transfer to a gelatin-coated plate to allow the cells to attach.
    Note: At this stage, cells can also be kept in suspension. In order to continue with static suspension culture, transfer the cells back to an ultra-low attachment plate.
  7. Change the medium with 3 ml RB medium after 2 days. The cultures will start to beat next day. Change the medium every 3 - 4 days with RB medium.

3. Differentiation of hPSCs as Spheroids in a Stirred Suspension Bioreactor

  1. Start experiment with undifferentiated spheroids cultured in static suspension for at least 3 passages (See Section 1.3.1). Add 10 µM ROCK inhibitor Y-27632 and incubate at 37 °C for 1 hr.
  2. Remove cells from incubator and transfer all spheroids into a 15 ml tube using a 5 ml pipette. Wash the well with 1 ml hESC medium to collect any remaining spheroids and add to the same 15 ml tube. Leave the cells for 5 min until spheroids have sedimented to form a loose pellet (DO NOT centrifuge). Aspirate the supernatant, being careful not to take any spheroids.
  3. Wash the cells by adding 1 ml PBS. Leave for 5 min until spheroids have sedimented to form a loose pellet (DO NOT centrifuge). Remove supernatant and add 0.5 ml 0.05% trypsin plus 0.53 mM EDTA. Incubate the cells for 4 - 5 min at 37 °C.
  4. Remove cells from the incubator and add 1 ml hESC medium. Using a p1,000 pipette, dissociate the cells into single cells, count the cells using Trypan blue and a hemocytometer. Resuspend to 2 × 105 viable cells/ml in conditioned medium supplemented with 100 ng/ml bFGF and 10 µM ROCK inhibitor Y-27632.
  5. Transfer 100 ml of cell suspension into the siliconized bioreactor flask with 1 pendulum (see Section 1.2.5). Start with 35 rpm agitation and after 24 hr increase to 40 rpm.
  6. After 48 hr, stop agitation for 5 - 10 min until all the spheroids have settled to the bottom of the flask. Replace half of the medium with conditioned medium supplemented with 100 ng/ml bFGF. Repeat the same process every 24 hr until day 5.
    Note: At this stage, undifferentiated spheroids with an average size of 175 ± 25 µm should be formed.
  7. Stop agitation for 5 - 10 min until all the spheroids have settled to the bottom of the flask. Transfer all spheroids to a 50 ml tube in a minimum amount of medium (less than 50 ml). Discard any remaining medium in the bioreactor flask, carefully ensuring no spheroids are remaining in the vessel.
  8. Leave for 5 - 10 min until all the spheroids have settled at the bottom of the 50 ml tube then carefully remove the supernatant without disrupting the loose cell pellet. Wash with 25 ml PBS with calcium and magnesium or RB medium, then leave again for 5 - 10 min until all the spheroids have settled at the bottom of the 50 ml tube to again form a loose pellet.
  9. Carefully remove the supernatant and add 40 ml RB medium supplemented with 12 µM CHIR99021, 10 µM Rock inhibitor and 0.1% poly vinyl alcohol (PVA). Resuspend the spheroids and transfer all cells back to the bioreactor flask. Add medium to make the total volume 100 ml. Restart agitation at 40 rpm for 24 hr.
    Note: RB medium is light sensitive. When working with RB medium keep the BSC in light switched off mode.
  10. Repeat Steps 3.7 - 3.8.
  11. Carefully remove the supernatant and add 40 ml RB medium supplemented with 0.1% PVA. Resuspend the spheroids and transfer all cells back to the bioreactor flask. Add medium to make the total volume 100 ml. Restart agitation at 40 rpm for 24 hr.
  12. Repeat Steps 3.7 - 3.8.
  13. Carefully remove the supernatant and add 40 ml RB medium supplemented with IWP2, purmorphamine and SB431542 (5 µM each). Resuspend the spheroids and transfer all cells back to the bioreactor flask. Add medium to make the total volume 100 ml. Restart agitation at 40 rpm for 2 days.
  14. Repeat Steps 3.7-3.8.
  15. Carefully remove the supernatant and add 40 ml RB medium only. Resuspend the spheroids and transfer all cells back to the bioreactor flask. Add medium to make the total volume 100 ml. Restart agitation at 40 rpm. Beating spheroids should be observed 48 - 72 hr later.
  16. Change half of the medium every 3 - 4 days with RB medium only by stopping the agitation for 5 - 10 min until all the spheroids have settled to the bottom of the vessel. Carefully remove 50 ml of medium without disturbing the spheroids and carefully replace with 50 ml of fresh RB medium.

4. Differentiation of hPSCs Using Cultures not Adapted to Single Cell Passaging

Note: This approach is specifically useful for the fast differentiation of a high number of hPSC lines without having to adapt to single-cell culturing techniques, an enormously labor-intensive and time consuming process. This technique is applicable to cell lines which are highly sensitive to enzymatic cell dissociation, such as newly established hPSC lines. 

  1. Start experiment with hPSCs cultured on MEF (as in Section 1.3.2) which are approximately 70 - 80% confluent. Remove any differentiated colonies using the stereomicroscope in the BSC.
  2. Remove medium and add 0.5 ml Dissociation Solution (DS). Incubate for 0.5 - 1 min until MEF cells have rounded up and the edges of hPSCs colonies become clear. Quickly remove DS and add 0.75 ml collagenase type IV (1 mg/ml).
  3. Incubate the cells for 5 - 15 min at 37 °C. Check the cells under the microscope during the incubation period, and when whole colonies are starting to lift off and detach, remove collagenase and add 1.5 ml hESC medium.
    Note: If after 15 min most of the colonies are still attached, put the cells back to incubator. Check the cells under the microscope every 5 min. Be careful not to leave the cells in collagenase for more than 30 min. If there are many floating colonies in the collagenase solution, do not remove the collagenase; just add 1 ml hESC medium.
  4. Using a p1,000 pipette, gently pipette colonies to detach as whole colonies. Do not break the colonies into small pieces. Transfer the cells into a 15 ml tube using a 5 ml pipette. Wash the well with 1 ml hESC medium to collect any remaining cells to add to the same tube. Leave for 5 min until all colonies have sedimented (DO NOT centrifuge).
  5. Carefully remove the supernatant and add 2 ml hESC medium supplemented with 100 ng/mL bFGF. Transfer the cells into an ultra-low attachment plate using 5 ml pipette (1 ml into each well of a 24-well plate and 3 ml into each well of a 6-well plate). Incubate at 37 °C/5% CO2 for at least 6 hr (maximum of 12 hr).
  6. During this time, prepare the required number of laminin-coated wells/plates for step 4.7. Recommended cell transfer ratios are 1 confluent well of a 6-well plate to 2 wells of a 24-well plate (or equivalent amount per surface area).
  7. After 6 hr, carefully transfer the colony aggregates into a 15 ml tube using 5 ml pipette. Wash the plate with RB medium to collect any remaining aggregates and add to the same 15 ml tube (0.5 ml per well for 24-well and 1 ml per well for 6-well plates). Wait 5 min until the aggregates sediment, then carefully aspirate the supernatant. Be careful not to disturb the loose pellet.
  8. Add 2 ml RB medium supplemented with 12 µM CHIR99021 and carefully transfer with a 5 ml pipette to your pre-prepared laminin coated wells (as in Section 1.2.4) to allow the cells to attach.
  9. After 24 hr, carefully remove the medium in each well and add 1 ml RB medium only.
    Note: Some of the aggregates will have only attached very weakly to the culture plate; therefore it is important to not remove any large aggregates which may have come loose during the medium change procedure. It is important, however, to remove as much of the small cell debris as possible.
  10. After 24 hr, carefully remove the medium and add RB medium supplemented with IWP2, purmorphamine and SB431542 (5 µM each).
  11. Change the medium after 2 days with RB medium only. Cells will start to beat next day.
  12. Change the medium every 3 - 4 days with RB medium only.

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Results

In order to establish a simple method for the large-scale differentiation of cardiomyocytes from hPSCs, we created a protocol in which cells were treated initially with a WNT/β-catenin activator (CHIR99021)16 and subsequently with inhibitors of the WNT/β-catenin and transforming growth factor-β (TGF-β) pathways (IWP216 and SB43154217, respectively) and finally an activator of the sonic hedgehog (SHH) pathway (purmorphamine)17

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Discussion

Cardiomyocytes derived from hPSCs are an extremely attractive source for use in human disease modeling, drug screening/toxicity testing and, perhaps in the future, regenerative therapies. One of the major hurdles to using these cells however, is the ability to provide enough high quality material for their effective use. Using our described protocol, we offer a method that overcomes this limitation.

Recently, synthetic small molecules targeting specific signaling pathways involved in cardiogen...

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Disclosures

The Victor Chang Cardiac Research Institute does not engage in, nor does it condone, the destruction of human embryos for research. Its contribution to this study was limited to work on human induced pluripotent stem cells.

The authors declare they have no competing financial interests.

Acknowledgements

This study was funded by grants provided from Royan Institute, Iranian Council of Stem Cell Research and Technology, the Iran National Science Foundation (INSF), the National Health and Medical Research Council of Australia (NHMRC; 354400), the National Heart Foundation of Australia/Heart Kids Australia (G11S5629), and the New South Wales Cardiovascular Research Network. HF was supported by a University International Postgraduate Scholarship from the University of New South Wales, Australia. RPH was supported by a NHMRC Australia Fellowship. The authors express their gratitude to the human subjects who participated in this research.

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Materials

NameCompanyCatalog NumberComments
Knockout DMEMLife Technologies10829018
Knockout Serum Replacement (KO-SR)Life Technologies10828028
GlutamaxLife Technologies35050061
MEM Non-essential Amino AcidsLife Technologies11140-050
β-MercaptoethanolLife Technologies21985-023
Basic Fibroblast Growth Factor (bFGF)Miltenyi Biotec130-093-843
RPMI1640Life Technologies11875093
DPBS, no calcium, no magnesiumLife Technologies14190144
DPBSLife Technologies14287072
Attachment Factor (AF)Life TechnologiesS006100
ECM GelSigma-AldrichE1270
LamininInvitrogen23017-015
DMEMLife Technologies11965-092                                                                                                       
Fatal Bovine Serum (FBS)Life Technologies16140-071
B27 minus insulinGibcoA18956-01
Penicillin/StreptomycinLife Technologies15070063
0.05% Trypsin/EDTALife Technologies25300-054
Collagenase Type IVLife Technologies17140-019
Calcium Chloride (CaCl2)Sigma-AldrichC7902
Mitomycin CBioaustralisBIA-M1183
CHIR99021Miltenyi Biotec130-104-172
IWP2Miltenyi Biotec130-105-335
SB431542Miltenyi Biotec130-095-561
PurmorphamineMiltenyi Biotec130-104-465
ROCK inhibitor Y-27632Miltenyi Biotec130-104-169
Ethylenediaminetetraacetic acid (EDTA)Sigma-AldrichE6758
Poly Vinyl Alcohol (PVA)Sigma-Aldrich363073
GelatinSigma-AldrichG1890
Trypan BlueBio-Rad145-0013
Accumax Innovative Cell Technologies Inc.AM105
Sigmacote Sigma-AldrichSL2 
CELLSPINIntegra Biosciences183 001
Spinner flask with 1 pendulum, 100 ml Integra Biosciences182 023
Mouse Embryonic Fibroblasts (MEF)Prepared in-house (or commercially available)
Human pluripotent stem cell (hPSC) linesPrepared in-house (or commercially available)

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Keyword Extraction CardiomyocytesHuman Pluripotent Stem CellsDifferentiation ProtocolHigh EfficiencyReproducibilityCardiac Disease ModelingHuman Induced Pluripotent Stem CellsCost effectiveHuman Embryonic Stem CellsSingle Cell PassagingSpheroidsPBSTrypsin EDTA

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