Erratum: Isolation of Human Hepatocytes by a Two-step Collagenase Perfusion Procedure

Summary

A correction was made to: Isolation of Human Hepatocytes by a Two-step Collagenase Perfusion Procedure.

The Final Concentration of EGTA in Solution 2 and the Final Concentration of Calcium chloride dihydrate in solutions 3 and 4 have been updated in Table 1.

Abstract

A correction was made to: Isolation of Human Hepatocytes by a Two-step Collagenase Perfusion Procedure

The changes listed below have been made to Table 1.

1). In the recipe for Solution 2, the Final Concentration of EGTA has been changed from:

EGTA

0.1mM

to:

EGTA

1mM

2). In the recipes for Solution 3 and 4, the Final Concentration of Calcium chloride dihydrate has been changed from:

Calcium chloride dihydrate

0.5µM

to:

Calcium chloride dihydrate

5mM

Protocol

A correction was made to: Isolation of Human Hepatocytes by a Two-step Collagenase Perfusion Procedure

The changes listed below have been made to Table 1.

1). In the recipe for Solution 2, the Final Concentration of EGTA has been changed from:

EGTA

0.1mM

to:

EGTA

1mM

2). In the recipes for Solution 3 and 4, the Final Concentration of Calcium chloride dihydrate has been changed from:

Calcium chloride dihydrate

0.5µM

to:

Calcium chloride dihydrate

5mM