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Abstract

Cancer Research

3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer

Published: September 13th, 2018

DOI:

10.3791/58345

1Departments of Internal Medicine, Hematology/Oncology Division, Rogel Cancer Center, University of Michigan Medical Center, 2Department of Urology, Division of GU Oncology, Rogel Cancer Center, University of Michigan Medical Center, 3Departments of Surgery and Pathology, Perlmutter Cancer Center, NYU Langone Health

Abstract

Bladder cancer is a significant health problem. It is estimated that more than 16,000 people will die this year in the United States from bladder cancer. While 75% of bladder cancers are non-invasive and unlikely to metastasize, about 25% progress to an invasive growth pattern. Up to half of the patients with invasive cancers will develop lethal metastatic relapse. Thus, understanding the mechanism of invasive progression in bladder cancer is crucial to predict patient outcomes and prevent lethal metastases. In this article, we present a three-dimensional cancer invasion model which allows incorporation of tumor cells and stromal components to mimic in vivo conditions occurring in the bladder tumor microenvironment. This model provides the opportunity to observe the invasive process in real time using time-lapse imaging, interrogate the molecular pathways involved using confocal immunofluorescent imaging and screen compounds with the potential to block invasion. While this protocol focuses on bladder cancer, it is likely that similar methods could be used to examine invasion and motility in other tumor types as well.

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Keywords 3D Cell Culture

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