University of Michigan Medical Center

4 ARTICLES PUBLISHED IN JoVE

Medicine

Production of Apolipoprotein C-III Knockout Rabbits using Zinc Finger Nucleases

Dongshan Yang¹, Jifeng Zhang¹, Jie Xu¹, Tianqing Zhu¹, Yanbo Fan¹, Jianglin Fan², Y. Eugene Chen¹

¹Center for Advanced Models for Translational Sciences and Therapeutics, Department of Internal Medicine, University of Michigan Medical Center, ²Department of Molecular Pathology, University of Yamanashi

Recent development in gene targeting tools makes production of knockout (KO) rabbits possible. In the present work, we generated five Apolipoprotein (Apo) C-III KO rabbits using Zinc Finger Nucleases (ZFN). This work demonstrated that ZFN is a highly efficient method to produce KO rabbits.

Cancer Research

3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer

Yin Wang¹, Mark L. Day², Diane M. Simeone³, Phillip L. Palmbos¹

¹Departments of Internal Medicine, Hematology/Oncology Division, Rogel Cancer Center, University of Michigan Medical Center, ²Department of Urology, Division of GU Oncology, Rogel Cancer Center, University of Michigan Medical Center, ³Departments of Surgery and Pathology, Perlmutter Cancer Center, NYU Langone Health

The processes governing bladder cancer invasion represent opportunities for biomarker and therapeutic development. Here we present a bladder cancer invasion model which incorporates 3-D culture of tumor spheroids, time-lapse imaging and confocal microscopy. This technique is useful for defining the features of the invasive process and for screening therapeutic agents.

Genetics

CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation

Linyuan Ma^*1, Lydia Jang^*1, Jian Chen², Jun Song¹, Dongshan Yang¹, Jifeng Zhang¹, Y. Eugene Chen¹, Jie Xu¹

¹Center for Advanced Models for Translational Sciences and Therapeutics, Department of Internal Medicine, University of Michigan Medical Center, ²Celetrix LLC

Presented here is a protocol for efficient CRISPR/Cas9 ribonucleoprotein-mediated gene editing in mammalian cells using tube electroporation.

Biology

Mouse Abdominal Aortic Aneurysm Model Induced by Perivascular Application of Elastase

Chao Xue¹, Guizhen Zhao¹, Yang Zhao¹, Y Eugene Chen¹, Jifeng Zhang¹

¹Department of Internal Medicine, Frankel Cardiovascular Center, University of Michigan, Ann Arbor

The present protocol describes a standardized surgical method for the elastase-induced AAA model through the direct application of elastase to the adventitia of infrarenal abdominal aorta in mice.