Immunology and Infection
Published: September 14th, 2018
Here we present a new method to accurately measure body temperature differences in passive systemic anaphylaxis (PSA) and food allergy mouse models using an infrared thermometer. This procedure has been accurately duplicated in previous PSA results.
Mouse body temperature measurement is of paramount importance for investigating allergies and anaphylactic symptoms. Rectal probes for temperature readings is common, and they have been proven to be accurate and invaluable in this regard. However, this method of temperature measurement requires the mice to be anesthetized in order to insert the probe without injury to the animal. This limits the ability to observe other phenotypes of the mouse simultaneously. In order to investigate other phenotypes while measuring temperatures, rectal probes are not ideal, and another method is desired. Here, we introduce a noninvasive method of temperature measurement that foregoes the requirement for mouse anesthesia while maintaining equal reliability to rectal probes in measuring body temperature. We use an infrared thermometer that detects body surface temperatures at ranges between 2 and 150 mm. This method of body temperature measurement is successful in reliably replicating temperature change trends during passive system anaphylaxis experiments in mice. We show that body surface temperatures are about 2.0 °C lower than rectal probe measurements, but the degree of temperature drop follows the same trend. Furthermore, we use the same technique to observe mice in a food allergy model to evaluate temperature and activity levels simultaneously.
Measurement of body temperature has been an essential part of monitoring the effects of anaphylactic symptoms in animal models1,2. Temperature differences have been traditionally measured by rectal probe thermometers in mice3,4. With these measurements, investigators have reliably portrayed differences in temperature among variables; however, this method is a time-consuming procedure and causes distress to mice, which can increase the body core temperature. Rectal probing can also cause mucosal tearing and infection3. Moreover, ....
All animal experiments were approved by the Animal Care and Use Committee of the La Jolla Institute for Allergy and Immunology.
1. Mouse Body Temperature Measurement During Anesthetization
Passive systemic anaphylaxis: For iv injection, 10 week old female BALB/c mice were anesthetized. Prior to the injection, we measured their body temperatures (Video 1) as described in step 1. Figure 1 shows the temperature trend of both populations after iv injection. The IgE-sensitized mouse showed a maximum temperature drop of 3.0 °C at 20 min, while the PBS control mouse had a maximum drop of 1.1 °C at 20 min7
The protocol described was established with the goal of measuring body temperature without the use of anesthesia. Despite its relative ease with which temperature readings can be obtained, there are several caveats that accommodate this technique, in addition to the more obvious effects such as handling stress and different ambient temperatures.
First, in order to maintain consistent temperature readings throughout the experiment, the location where the temperature is being measured must be pr.......
|Non-contact infrared thermometer
|Anti-dinitrophenyl (DNP) IgE
|PrecisionGlide 30G needle
|PrecisionGlide 26G needle
|1 mL syringe
|Dinitrophenyl - human serum albumin
|Ovalbumin from chicken egg white
|Animal Feeding Needles, disposable
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