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Abstract

Immunology and Infection

Using Robotic Systems to Process and Embed Colonic Murine Samples for Histological Analyses

Published: January 7th, 2019

DOI:

10.3791/58654

1Pathology Unit, Fondazione IRCCS Cà Granda, Ospedale Maggiore Policlinico, 2Department of Experimental Oncology, European Institute of Oncology, 3Gastroenterology and Endoscopy Unit, Fondazione IRCCS Cà Granda, Ospedale Maggiore Policlinico, 4Department of Pathophysiology and Transplantation, Università degli Studi di Milano

* These authors contributed equally

ERRATUM NOTICE

Important: There has been an erratum issued for this article. Read more …

Abstract

The understanding of human diseases has been greatly expanded thanks to the study of animal models. Nonetheless, histopathological evaluation of experimental models needs to be as rigorous as that applied for human samples. Indeed, drawing reliable and accurate conclusions is critically influenced by the quality of tissue section preparation. Here, we describe a protocol for histopathological analysis of murine tissues that implements several automated steps during the procedure, from the initial preparation to the paraffin embedding of the murine samples. The reduction of methodological variables through rigorous protocol standardization from automated procedures contributes to increased overall reliability of murine pathological analysis. Specifically, this protocol describes the utilization of automated processing and embedding robotic systems, routinely used for the tissue processing and paraffin embedding of human samples, to process murine specimens of intestinal inflammation. We conclude that the reliability of histopathological examination of murine tissues is significantly increased upon introduction of standardized and automated techniques.

Erratum

Erratum: Using Robotic Systems to Process and Embed Colonic Murine Samples for Histological Analyses

An erratum was issued for: Using Robotic Systems to Process and Embed Colonic Murine Samples for Histological Analyses.  An author affiliation was updated.

The affiliation for Claudia Burrello and Federica Facciotti was updated from:

Department of Experimental Oncology, European Institute of Oncology

to:

Department of Experimental Oncology, IEO, European Institute of Oncology IRCCS

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Keywords Murine Colon Tissue

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