Erratum: Stereotaxic Viral Injection and Gradient-Index Lens Implantation for Deep Brain In Vivo Calcium Imaging

Summary

An erratum was issued for: Stereotaxic Viral Injection and Gradient-Index Lens Implantation for Deep Brain In Vivo Calcium Imaging. The Discussion was updated.

Abstract

An erratum was issued for: Stereotaxic Viral Injection and Gradient-Index Lens Implantation for Deep Brain In Vivo Calcium Imaging. The Discussion was updated.

The following paragraph was added to the end of the Discussion:

Although a chunk of brain tissue is removed unilaterally during the GRIN lens implantation step, we did not observe any obvious behavior deficits^7,12. The weight of the miniscope is around 2 grams and the cable is custom-designed to make it light and to ensure that the mouse can easily carry it. The miniscope and cable are only attached to the animal prior to in vivo imaging and detached after imaging. The entire imaging process usually takes no longer than 30 minutes. Therefore, these instrumentations do not prevent the mouse from freely behaving. The miniscope installation and deinstallation steps need a brief anesthesia (less than 2 minutes) with isoflurane for the purpose of animal restraining. We typically let the mouse recover from the brief exposure of isoflurane for 30 minutes before performing in vivo imaging. We have performed miniscope in vivo calcium imaging once per week for a few weeks without noticing any impact on mouse health and mouse social behavior¹².

Protocol

An erratum was issued for: Stereotaxic Viral Injection and Gradient-Index Lens Implantation for Deep Brain In Vivo Calcium Imaging. The Discussion was updated.

The following paragraph was added to the end of the Discussion:

Although a chunk of brain tissue is removed unilaterally during the GRIN lens implantation step, we did not observe any obvious behavior deficits^7,12. The weight of the miniscope is around 2 grams and the cable is custom-designed to make it light and to ensure that the mouse can easily carry it. The miniscope and cable are only attached to the animal prior to in vivo imaging and detached after imaging. The entire imaging process usually takes no longer than 30 minutes. Therefore, these instrumentations do not prevent the mouse from freely behaving. The miniscope installation and deinstallation steps need a brief anesthesia (less than 2 minutes) with isoflurane for the purpose of animal restraining. We typically let the mouse recover from the brief exposure of isoflurane for 30 minutes before performing in vivo imaging. We have performed miniscope in vivo calcium imaging once per week for a few weeks without noticing any impact on mouse health and mouse social behavior¹².