Published: April 14th, 2023
This protocol demonstrates a unique mouse model of asphyxia cardiac arrest that does not require chest compression for resuscitation. This model is useful for monitoring and imaging the dynamics of brain physiology during cardiac arrest and resuscitation.
Most cardiac arrest (CA) survivors experience varying degrees of neurologic deficits. To understand the mechanisms that underpin CA-induced brain injury and, subsequently, develop effective treatments, experimental CA research is essential. To this end, a few mouse CA models have been established. In most of these models, the mice are placed in the supine position in order to perform chest compression for cardiopulmonary resuscitation (CPR). However, this resuscitation procedure makes the real-time imaging/monitoring of brain physiology during CA and resuscitation challenging. To obtain such critical knowledge, the present protocol presents a mouse asphyxia CA model that does not require the chest compression CPR step. This model allows for the study of dynamic changes in blood flow, vascular structure, electrical potentials, and brain tissue oxygen from the pre-CA baseline to early post-CA reperfusion. Importantly, this model applies to aged mice. Thus, this mouse CA model is expected to be a critical tool for deciphering the impact of CA on brain physiology.
Cardiac arrest (CA) remains a global public health crisis1. More than 356,000 out-of-hospital and 290,000 in-hospital CA cases are reported annually in the US alone, and most CA victims are over 60 years old. Notably, post-CA neurologic impairments are common among survivors, and these represent a major challenge for CA management2,3,4,5. To understand post-CA brain pathologic changes and their effects on neurologic outcomes, various neurophysiologic monitoring and brain tissue monitoring techniq....
All the procedures described here were conducted in accordance with the National Institutes of Health (NIH) guidelines for the care and use of animals in research, and the protocol was approved by the Duke Institute of Animal Care and Use Committee (IACUC). C57BL/6 male and female mice aged 8-10 weeks old were used for the present study.
1. Surgical preparation
To induce CA, the mouse was anesthetized with 1.5% isoflurane and ventilated with 100% nitrogen. This condition led to severe bradycardia in 45 s (Figure 1). Following 2 min of anoxia, the heart rate dramatically reduced (Figure 2), the blood pressure decreased below 20 mmHg, and the cerebral blood flow ceased completely (Figure 1). As the isoflurane was turned off, the body temperature was no longer managed and slowly dropped .......
In experimental CA studies, asphyxia, potassium chloride injections, or electrical current-derived ventricular fibrillation have been used to induce CA16,17,18,19,20,21,22,23. Normally, CPR is required for resuscitation in these CA models, especially in mic.......
The authors thank Kathy Gage for her editorial support. This study was supported by funds from the Department of Anesthesiology (Duke University Medical Center), American Heart Association grant (18CSA34080277), and National Institutes of Health (NIH) grants (NS099590, HL157354, NS117973, and NS127163).....
|Animal Bio Amp
|Heparin sodium injection, USP
|Laser Doppler perfusion monitor
|Laser speckle imaging system
|Lubricant eye ointment
|Bausch + Lomb
|Mouse rectal probe
|29 Ga, 1.5 mm socket
|Optic plastic fibre
|2.5 mm Speculum
|Injectable 50 mg/ml
|Small animal ventilator
|Triple antibioric & pain relief
|0.9% sodium chroride
|1 mL plastic syringe
|4-0 silk suture
|Black braided silk
|6-0 nylon suture
|8.4% sodium bicarbonate Inj., USP
|20 G IV catheter
|20GA 1.6 IN
|30 G PrecisionGlide needle
|30 G X 1/2
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