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Medicine

Real-Time Detection of Ferulic Acid Effects on Rat Left Ventricle Using Pressure-Volume Conductivity Catheter

Published: January 12th, 2024

DOI:

10.3791/65858

1School of Basic Medical Sciences, Chengdu University of Traditional Chinese Medicine, 2Innovative Institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine, 3Research Institute of Integrated TCM & Western Medicine, Chengdu University of Traditional Chinese Medicine

This protocol describes a method for measuring left ventricular pressure and volume using the pressure-volume conductance technique. This method enables continuous real-time monitoring of the effects of drugs on the heart.

Decreased cardiac function can have a negative impact on other organs. The left ventricular pressure-volume relationship is considered to be a valid method for evaluating cardiac function. Real-time monitoring of cardiac function is important for drug evaluation. Under closed-chest conditions, the miniature transducer, which is an important component of the pressure-volume catheter, enters the left ventricle of the rat through the right carotid artery. The device visualizes the changes in cardiac function during the experiment in the form of a pressure-volume loop. The actual volume of the ventricle is calculated by altering the conductivity of the blood by injecting 50 µL of a 20% sodium chloride solution into the rat's left jugular vein. The actual volume of the rat's ventricular cavity is calculated by measuring the conductivity of the blood in a known volume using a pressure-volume conductance catheter. This protocol allows for continuous observation of the effects of drugs on the heart and will promote the rationale for the use of specialty ethnic drugs in cardiovascular disease.

Cardiovascular disease has the highest mortality rate in the world1. Its causes include coronary artery stenosis (myocardial ischemia), coronary artery blockage (myocardial infarction), and ischemia-reperfusion injury2. As the heart is in a constant systolic and diastolic cycle, it is one of the most energy-demanding parts of the body. Therefore, when the coronary arteries have difficulty maintaining sufficient energy and oxygen, cardiac function inevitably decreases, which has a negative impact on other organs3,4. The heart is a powerhouse in the circulatory sys....

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The animal protocol was reviewed and approved by the Chengdu University of Traditional Chinese Medicine Experimental Animal Welfare Ethics Committee (Record No. 2023-04). Male Sprague Dawley (SD) rats (280 ± 20 g, 8-10 weeks old) were used for the present study. The rats were kept in an animal chamber and were free to drink and eat.

1. Solution preparation

  1. Prepare 0.9% NaCl solution to be used to keep the work area adequately moist.
  2. To prepare 20% hy.......

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Each test (n = 3) was predicated on the entry of a pressure-volume conductivity catheter into the left ventricle. There are significant signal changes, such as a marked increase in the range of pressure, as the catheter enters the left ventricle from the carotid artery (Figure 1). Graphical analysis of the pressure-volume relationship is completed by plotting the volume (µL) on the Y-axis and the pressure (mmHg) on the X-axis. Rat left ventricular pressure was within 10-105 mmHg, and co.......

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It is essential to adopt a rational dosing strategy for different states of cardiac function. The pressure-volume conductance catheter technique is the most intuitive way to study left ventricular function5. This method enables the effects of drugs on cardiac function to be studied from a whole perspective. We describe the various stages of the experiment in detail. This will provide some facility for the study of heart function.

The pressure volume conductivity cathete.......

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This work was supported by the Sichuan Provincial Major R&D Project (2022YFS043) and the Chengdu University of Traditional Chinese Medicine Youth Foundation Advancement Talent Special Project (QJJJ2022029).

....

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NameCompanyCatalog NumberComments
1 mL syringeSartorius AG, Germany-
Animal temperature maintainerRayward Life Technology Co., Ltd69020
Dual Bio AmpMillar, Inc., USADA-100
Enzyme-Active Powdered DetergentAlconox Inc., USA1104
Ferulic acid Macklin Biochemical Co., Ltd,Shanghai, ChinaF900027
Mikro-Tip Catheter Transducers, SPR-838NRMillar, Inc., USASPR-838NR
Millar Miro-Tip Pressure Volume (MPVS) UltraMillar, Inc., USASPR-869
Pet electric clippersJinyun County New Concept Home Supplies Co., Ltd. -
Power Lab 8 / 35Millar, Inc., USAPL3508
Sodium Chloride, NaCl Kelong Chemical Reagent, Chengdu, ChinaKX829463
Veet hair removal creamShanghai Songqi E-commerce Co., Ltd.3226470

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