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Abstract

Bioengineering

Cell Patterning Using Magnetic-Archimedes Strategy

Published: February 2nd, 2024

DOI:

10.3791/66063

1Department of Cardiology of The Second Affiliated Hospital, Zhejiang University School of Medicine, 2State Key Laboratory of Transvascular Implantation Devices, 3Cardiovascular Key Laboratory of Zhejiang Province

Abstract

Cell patterning, allowing precise control of cell positioning, presents a unique advantage in the study of cell behavior. In this protocol, a cell patterning strategy based on the Magnetic-Archimedes (Mag-Arch) effect is introduced. This approach enables precise control of cell distribution without the use of ink materials or labeling particles. By introducing a paramagnetic reagent to enhance the magnetic susceptibility of the cell culture medium, cells are repelled by magnets and arrange themselves into a pattern complementary to the magnet sets positioned beneath the microfluidic substrate.

In this article, detailed procedures for cell patterning using the Mag-Arch-based strategy are provided. Methods for patterning single-cell types as well as multiple cell types for co-culture experiments are offered. Additionally, comprehensive instructions for fabricating microfluidic devices containing channels for cell patterning are provided. Achieving this feature using parallel methods is challenging but can be done in a simplified and cost-effective manner. Employing Mag-Arch-based cell patterning equips researchers with a powerful tool for in vitro research.

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Keywords Cell Patterning

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