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Abstract

Biology

Non-invasive Assay for Chlorophyll Biosynthesis Kinetics Determination during Early Stages of Arabidopsis De-etiolation

Published: January 12th, 2024

DOI:

10.3791/66087

1CEITEC - Central European Institute of Technology, Masaryk University, 2National Centre for Biomolecular Research, Faculty of Science, Masaryk University, 3Institute of Food Biotechnology and Genomics, National Academy of Sciences of Ukraine, 4Labdeers s.r.o, 5Photon Systems Instruments, Prumyslova

* These authors contributed equally

Abstract

Chlorophyll biosynthesis is a hallmark of de-etiolation, one of the most dramatic stages in the plant life cycle. The tightly controlled and highly dynamic process of chlorophyll biosynthesis is triggered during the shift from the dark to the light in flowering plants. At the moment when etiolated seedlings are exposed to the first traces of sunlight, rapid (in order of seconds) conversion of protochlorophyllide into chlorophyllide is mediated by unique light-accepting protein complexes, leading via subsequent metabolic steps to the production of fully functional chlorophyll. Standard techniques for chlorophyll content analysis include pigment extraction from detached plant tissues, which does not apply to studying such fast processes. To investigate chlorophyll kinetics in vivo with high accuracy and spatiotemporal resolution in the first hours after light-induced de-etiolation, an instrument and protocol were developed. Here, we present a detailed procedure designed for statistically robust quantification of chlorophyll in the early stages of Arabidopsis de-etiolation.

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Keywords Chlorophyll Biosynthesis

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