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4D Light-sheet Imaging of Zebrafish Cardiac Contraction
In This Article
Summary
This protocol utilizes light-sheet imaging to investigate cardiac contractile function in zebrafish larvae and gain insights into cardiac mechanics through cell tracking and interactive analysis.
Abstract
Zebrafish is an intriguing model organism known for its remarkable cardiac regeneration capacity. Studying the contracting heart in vivo is essential for gaining insights into structural and functional changes in response to injuries. However, obtaining high-resolution and high-speed 4-dimensional (4D, 3D spatial + 1D temporal) images of the zebrafish heart to assess cardiac architecture and contractility remains challenging. In this context, an in-house light-sheet microscope (LSM) and customized computational analysis areĀ used to overcome these technical limitations. This strategy, involving LSM system construction, retrospective synchronization, single cell tracking, and user-directed analysis, enables one to investigate the micro-structure and contractile function across the entire heart at the single-cell resolution in the transgenic Tg(myl7:nucGFP) zebrafish larvae. Additionally, we are able to further incorporate microinjection of small molecule compounds to induce cardiac injury in a precise and controlled manner. Overall, this framework allows one to track physiological and pathophysiological changes, as well as the regional mechanics at the single-cell level during cardiac morphogenesis and regeneration.
Introduction
The zebrafish (Danio rerio) is a widely used model organism for studying cardiac development, physiology, and repair due to its optical transparency, genetic tractability, and regenerative capacity1,2,3,4. Upon myocardial infarction, while structural and functional changes impact the cardiac ejection and hemodynamics, technical limitations continue to hinder the ability to investigate the dynamic process during cardiac regeneration with the high spatiotemporal resolution. For example, conventional imaging methods, such as confocal m....
Protocol
Approval for this study was granted by the Institutional Animal Care and Use Committee (IACUC) of the University of Texas at Dallas, under protocol number #20-07. Tg(myl7:nucGFP) transgenic zebrafish larvae12 were used for the present study. All data acquisition and image post-processing were carried out using open-source software or platforms with research or educational licenses. The resources are available from the authors upon reasonable request.
1. Z.......
Representative Results
The current protocol consists of three main steps: zebrafish preparation and microinjection, light-sheet imaging and 4D image reconstruction, and cell tracking and VR interaction. Adult zebrafish were allowed to mate, the fertilized eggs were collected, and performed microinjection as needed for the proposed experiments (Figure 1). This step provides an entry point to explore zebrafish applications in the investigation of cardiac development and regeneration, and it also plays a crucial role.......
Discussion
The integration of the zebrafish model with engineering methods holds immense potential for the in vivo exploration of myocardial infarction, arrhythmias, and congenital heart defects. Leveraging its optical transparency, regenerative capacity, and genetic and physiological similarities to humans, zebrafish embryos and larvae have become extensively utilized in research1,2,4. The superior spatiotemporal resolution, mini.......
Acknowledgements
We express our gratitude to Dr. Caroline Burns at Boston Children's Hospital for generously sharing the transgenic zebrafish. We thank Ms. Elizabeth Ibanez for her help in husbanding zebrafish at UT Dallas. We also appreciate all the constructive comments provided by D-incubator members at UT Dallas. This work was supported by NIH R00HL148493 (Y.D.), R01HL162635 (Y.D.), and UT Dallas STARS program (Y.D.).
....Materials
| Name | Company | Catalog Number | Comments |
| RESOURCE | SOURCE/Reference | IDENTIFIER | |
| Animal models | |||
| Tg(myl7:nucGFP) transgenic zebrafish | Burns Lab in Boston Children's Hospital | ZDB-TGCONSTRCT-070117-49 | |
| Software and algorithms | |||
| MATLAB | The MathWorks Inc. | R2023a | |
| LabVIEW | National Instruments Corporation | 2017 SP1 | |
| HCImage Live | Hamamatsu Photonics | 4.6.1.2 | |
| Python | The Python Software Foundation | 3.9.0 | |
| Fiji-ImageJ | Schneider et al.18 | 1.54f | |
| 3DeeCellTracker | Chentao Wen et al.15 | v0.5.2 | |
| Unity | Unity Software Inc. | 2020.3.2f1 | |
| Amira | Thermo Fisher Scientific | 2021.2 | |
| 3D Slicer | Andriy Fedorov et al.17 | 5.2.1 | |
| ITK SNAP | Paul A Yushkevich et al.16 | 4 | |
| Light-sheet system | |||
| Cylindrical lens | Thorlabs | ACY254-050-A | |
| 4X Illumination objective | Nikon | MRH00045 | |
| 20X Detection objective | Olympus | 1-U2M585 | |
| sCMOS camera | Hamamatsu | C13440-20CU | |
| Motorized XYZ stage | Thorlabs | PT3/M-Z8 | |
| Two-axis tilt stage | Thorlabs | GN2/M | |
| Rotation stepper motor | Pololu | 1474 | |
| Fluorescent beads | Spherotech | FP-0556-2 | |
| 473nm DPSS Laser | Laserglow | R471003GX | |
| 532nm DPSS laser | Laserglow | R531003FX | |
| Microinjector and vacuum pump | |||
| Microinjector | WPI | PV850 | |
| Vacuum pump | Welch | 2522B-01 | |
| Pre-Pulled Glass Pipettes | WPI | TIP10LT | |
| Capillary tip for gel loading | Bio-Rad | 2239912 | |
| Virtual reality hardware | |||
| VR headset | Meta | Quest 2 | |
| 30mg/L PTU solution | |||
| PTU | Sigma-Aldrich | P7629 | |
| 1X E3 working solution | - | - | |
| 1% Agarose | - | - | |
| Low-melt agarose | Thermo Fisher | 16520050 | |
| Deionized water | - | - | |
| 10g/L Tricaine stock solution | |||
| Tricaine | Syndel | SYNC-M-GR-US02 | |
| Deionized water | - | - | |
| Sodium bicarbonate | Sigma-Aldrich | S6014 | |
| 150mg/L Tricaine working solution | |||
| 10g/L Tricaine stock solution | - | - | |
| Deionized water | - | - | |
| 60X E3 stock solution | |||
| Sodium Chloride | Lab Animal Resource Center (LARC), The University of Texas at Dallas | NaCl | |
| Potassium Chloride | - | KCL | |
| Calcium Chloride Dihydrate | - | CaCL2 x 2H2O | |
| Magnesium Sulfate Heptahydrate | - | MgSO4 x 7H2O | |
| RO Water | - | - | |
| 1X E3 working solution | |||
| 60X E3 stock solution | Lab Animal Resource Center (LARC), The University of Texas at Dallas | - | |
| RO Water | - | - | |
| 1% Methylene Blue (optional)Ā | - | C16H18ClN3S |
References
- Power, R. M., Huisken, J. A guide to light-sheet fluorescence microscopy for multiscale imaging. Nat. Methods. 14 (4), 360-373 (2017).
- Liu, J., Stainier, D. Y. R. Zebrafish in the study of early cardiac development.
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