A subscription to JoVE is required to view this content. Sign in or start your free trial.
Method Article
Preparation and Fractionation of Xenopus laevis Egg Extracts
Summary
Crude and fractionated Xenopus egg extracts can be used to provide ingredients for reconstituting cellular processes for morphological and biochemical analysis. Egg lysis and differential centrifugation are used to prepare the crude extract which in turn in used to prepare fractionated extracts and light membrane preparations.
Abstract
Crude and fractionated Xenopus egg extracts can be used to provide ingredients for reconstituting cellular processes for morphological and biochemical analysis. Egg lysis and differential centrifugation are used to prepare the crude extract which in turn in used to prepare fractionated extracts and light membrane preparations.
Protocol
The complete text protocol for this experimental approach is available in Current Protocols in Cell Biology.
Disclosures
The authors have nothing to disclose.
References
Reprints and Permissions
Request permission to reuse the text or figures of this JoVE article
Request PermissionThis article has been published
Video Coming Soon
Copyright © 2025 MyJoVE Corporation. All rights reserved