Sakae Tsuda

Assignments of 1H, 13C, and 15N resonances of human lysozyme at 4 degrees C.

Alternate conformations observed in catalytic serine of Bacillus subtilis lipase determined at 1.3 A resolution.

A novel plant defensin-like gene of winter wheat is specifically induced during cold acclimation.

Production and characterization of recombinant tachycitin, the Cys-rich chitin-binding protein.

Low-temperature-induced structural changes in human lysozyme elucidated by three-dimensional NMR spectroscopy.

Type II antifreeze protein from a mid-latitude freshwater fish, Japanese smelt (Hypomesus nipponensis).

Artificial multimers of the type III antifreeze protein. Effects on thermal hysteresis and ice crystal morphology.

Crystallization and preliminary X-ray crystallographic study on a xyloglucan-specific exo-beta-glycosidase, oligoxyloglucan reducing-end specific cellobiohydrolase.

Antifreeze glycoproteins: elucidation of the structural motifs that are essential for antifreeze activity.

Tandem repeat of a seven-bladed beta-propeller domain in oligoxyloglucan reducing-end-specific cellobiohydrolase.

Co-operative effect of the isoforms of type III antifreeze protein expressed in Notched-fin eelpout, Zoarces elongatus Kner.

A part of ice nucleation protein exhibits the ice-binding ability.

Thermal stabilization of Bacillus subtilis family-11 xylanase by directed evolution.

Crystallization and preliminary X-ray crystallographic analysis of Ca2+-independent and Ca2+-dependent species of the type II antifreeze protein.

Activity of a two-domain antifreeze protein is not dependent on linker sequence.

The structural basis for the exo-mode of action in GH74 oligoxyloglucan reducing end-specific cellobiohydrolase.

Effect of annealing time of an ice crystal on the activity of type III antifreeze protein.

Two domains of RD3 antifreeze protein diffuse independently.

Hypothermic preservation effect on mammalian cells of type III antifreeze proteins from notched-fin eelpout.

Crystal structure and mutational analysis of Ca2+-independent type II antifreeze protein from longsnout poacher, Brachyopsis rostratus.

Fully active QAE isoform confers thermal hysteresis activity on a defective SP isoform of type III antifreeze protein.

One-pot synthesis of cyclic antifreeze glycopeptides.

Crystal structure and enhanced activity of a cutinase-like enzyme from Cryptococcus sp. strain S-2.

Construction of time-lapse scanning electrochemical microscopy with temperature control and its application to evaluate the preservation effects of antifreeze proteins on living cells.

The crystal structure of a xyloglucan-specific endo-beta-1,4-glucanase from Geotrichum sp. M128 xyloglucanase reveals a key amino acid residue for substrate specificity.

Comparison of functional properties of two fungal antifreeze proteins from Antarctomyces psychrotrophicus and Typhula ishikariensis.

Synthetic study and structural analysis of the antifreeze agent xylomannan from Upis ceramboides.

Ice-binding site of snow mold fungus antifreeze protein deviates from structural regularity and high conservation.

Engineering a naturally inactive isoform of type III antifreeze protein into one that can stop the growth of ice.

NMR structure note: a defective isoform and its activity-improved variant of a type III antifreeze protein from Zoarces elongates Kner.

A simple medium enables bovine embryos to be held for seven days at 4°C.

Microwave-assisted solid-phase synthesis of antifreeze glycopeptides.

Cold adaptation of fungi obtained from soil and lake sediment in the Skarvsnes ice-free area, Antarctica.

Antifreeze protein prolongs the life-time of insulinoma cells during hypothermic preservation.

Annealing condition influences thermal hysteresis of fungal type ice-binding proteins.

Identification of a Novel LEA Protein Involved in Freezing Tolerance in Wheat.

Antifreeze protein activity in Arctic cryoconite bacteria.