Karl E. Griswold

Effects of codon usage versus putative 5'-mRNA structure on the expression of Fusarium solani cutinase in the Escherichia coli cytoplasm.

pH sensing agar plate assays for esterolytic enzyme activity.

Enhanced crossover SCRATCHY: construction and high-throughput screening of a combinatorial library containing multiple non-homologous crossovers.

Evolution of highly active enzymes by homology-independent recombination.

Direct selection of trans-acting ligase ribozymes by in vitro compartmentalization.

The evolution of catalytic efficiency and substrate promiscuity in human theta class 1-1 glutathione transferase.

Quantifying and resolving multiple vector transformants in S. cerevisiae plasmid libraries.

Protein fragment swapping: a method for asymmetric, selective site-directed recombination.

Optimization algorithms for functional deimmunization of therapeutic proteins.

Enhanced antimicrobial activity of engineered human lysozyme.

The monoclonal myth.

Genetically engineered alginate lyase-PEG conjugates exhibit enhanced catalytic function and reduced immunoreactivity.

Crystal structure of a charge engineered human lysozyme having enhanced bactericidal activity.

Optimization of therapeutic proteins to delete T-cell epitopes while maintaining beneficial residue interactions.

Optimization of combinatorial mutagenesis.

Design and analysis of immune-evading enzymes for ADEPT therapy.

Alginate lyase exhibits catalysis-independent biofilm dispersion and antibiotic synergy.

Engineering Escherichia coli for soluble expression and single step purification of active human lysozyme.

Structure-based redesign of proteins for minimal T-cell epitope content.

Structure-guided deimmunization of therapeutic proteins.

Molecular sensing with magnetic nanoparticles using magnetic spectroscopy of nanoparticle Brownian motion.

A high-throughput screen for antibiotic drug discovery.

Bioengineered lysozyme reduces bacterial burden and inflammation in a murine model of mucoid Pseudomonas aeruginosa lung infection.

Gene and protein sequence optimization for high level production of fully active and aglycosylated lysostaphin in Pichia pastoris.

Bioengineered lysozyme in combination therapies for Pseudomonas aeruginosa lung infections.