application-rnai-heat-shock-induced-transcription-factor-expression

Application of RNAi and Heat-shock-induced Transcription Factor Expression to Reprogram Germ Cells to Neurons in <em>C. elegans</em>

Studying the cell biological processes during converting the identities of specific cell types provides important insights into mechanism that maintain ...

Max Delbrück Center for Molecular Medicine in the Helmholtz Association

The identity of individual cell types in a multicellular organism appears to be continuously maintained through active processes but is not irreversible. Changes in the identity of individual cell types can be brought about through ectopic mis-expression of regulatory factors, but in a number of cases also occurs in normal development. I will review here these natural cellular reprogramming processes occurring in the invertebrate model organisms Caenorhabditis elegans and Drosophila melanogaster. Furthermore, I will discuss the issue of why only certain cell types can be converted during induced reprogramming processes evoked by ectopic expression of regulatory factors and how recent work in model systems have shown that this cellular context-dependency can be manipulated.

Cellular reprogramming processes in Drosophila and C. elegans.

Affinity purification followed by mass spectrometry (AP/MS) is a widely used approach to identify protein interactions and complexes. In multicellular organisms, the accurate identification of protein complexes by AP/MS is complicated by the potential heterogeneity of complexes in different tissues. Here, we present an in vivo biotinylation-based approach for the tissue-specific purification of protein complexes from Caenorhabditis elegans. Tissue-specific biotinylation is achieved by the expression in select tissues of the bacterial biotin ligase BirA, which biotinylates proteins tagged with the Avi peptide.

Baris Tursun

Berlin Institute for Medical Systems Biology

Max Delbrück Center for Molecular Medicine in the Helmholtz Association

Application of RNAi and Heat-shock-induced Transcription Factor Expression to Reprogram Germ Cells to Neurons in C. elegans

Baris Tursun

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Berlin Institute for Medical Systems Biology

Max Delbrück Center for Molecular Medicine in the Helmholtz Association

Application of RNAi and Heat-shock-induced Transcription Factor Expression to Reprogram Germ Cells to Neurons in C. elegans

Berlin Institute for Medical Systems Biology