Mario Roederer

Human immunodeficiency virus type 1 neutralization measured by flow cytometric quantitation of single-round infection of primary human T cells.

Optimized determination of T cell epitope responses.

Measuring containment of viable infectious cell sorting in high-velocity cell sorters.

Toll-like receptor ligands modulate dendritic cells to augment cytomegalovirus- and HIV-1-specific T cell responses.

Sensitive and viable identification of antigen-specific CD8+ T cells by a flow cytometric assay for degranulation.

Flow cytometric analysis of vaccine responses: how many colors are enough?

T-cell dynamics during acute SIV infection.

The functional profile of primary human antiviral CD8+ T cell effector activity is dictated by cognate peptide concentration.

Seventeen-colour flow cytometry: unravelling the immune system.

Vaccination in humans generates broad T cell cytokine responses.

Massive infection and loss of memory CD4+ T cells in multiple tissues during acute SIV infection.

Infectious molecular clones from a simian immunodeficiency virus-infected rapid-progressor (RP) macaque: evidence of differential selection of RP-specific envelope mutations in vitro and in vivo.

HIV nonprogressors preferentially maintain highly functional HIV-specific CD8+ T cells.

Toll-like receptor agonists influence the magnitude and quality of memory T cell responses after prime-boost immunization in nonhuman primates.

Immune protection of nonhuman primates against Ebola virus with single low-dose adenovirus vectors encoding modified GPs.

Vaccination preserves CD4 memory T cells during acute simian immunodeficiency virus challenge.

Amine reactive dyes: an effective tool to discriminate live and dead cells in polychromatic flow cytometry.

Systemic vaccination prevents the total destruction of mucosal CD4 T cells during acute SIV challenge.

Phase 1 safety and immunogenicity evaluation of a multiclade HIV-1 candidate vaccine delivered by a replication-defective recombinant adenovirus vector.

Phase 1 safety and immunogenicity evaluation of a multiclade HIV-1 DNA candidate vaccine.

Safety, immunogenicity and efficacy of modified vaccinia Ankara (MVA) against Dryvax challenge in vaccinia-naïve and vaccinia-immune individuals.

Acquisition of direct antiviral effector functions by CMV-specific CD4+ T lymphocytes with cellular maturation.

Differential susceptibility of leukocyte subsets to cytotoxic T cell killing: implications for HIV immunopathogenesis.

Increased immunofluorescence sensitivity using 532 nm laser excitation.

Safety and immunogenicity of a Gag-Pol candidate HIV-1 DNA vaccine administered by a needle-free device in HIV-1-seronegative subjects.

Live-cell assay to detect antigen-specific CD4+ T-cell responses by CD154 expression.

Intracellular cytokine optimization and standard operating procedure.

Quality assurance for polychromatic flow cytometry.

SIV-specific CD8+ T cells express high levels of PD1 and cytokines but have impaired proliferative capacity in acute and chronic SIVmac251 infection.

Estimating the infectivity of CCR5-tropic simian immunodeficiency virus SIV(mac251) in the gut.

Immunization with vaccinia virus induces polyfunctional and phenotypically distinctive CD8(+) T cell responses.

Multifunctional TH1 cells define a correlate of vaccine-mediated protection against Leishmania major.

Optimizing a multicolor immunophenotyping assay.

A West Nile virus DNA vaccine induces neutralizing antibody in healthy adults during a phase 1 clinical trial.

Polyfunctional T cell responses are a hallmark of HIV-2 infection.

How many events is enough? Are you positive?

T-cell quality in memory and protection: implications for vaccine design.

Multiparameter FACS analysis.

IL-15 expands unconventional CD8alphaalphaNK1.1+ T cells but not Valpha14Jalpha18+ NKT cells.

Polychromatic plots: graphical display of multidimensional data.

Antiretroviral therapy prior to acute viral replication preserves CD4 T cells in the periphery but not in rectal mucosa during acute simian immunodeficiency virus infection.

Compensation in flow cytometry.

Gating-ML: XML-based gating descriptions in flow cytometry.

A SARS DNA vaccine induces neutralizing antibody and cellular immune responses in healthy adults in a Phase I clinical trial.

Techniques to improve the direct ex vivo detection of low frequency antigen-specific CD8+ T cells with peptide-major histocompatibility complex class I tetramers.

Emerging concepts in the immunopathogenesis of AIDS.

High avidity myeloid leukemia-associated antigen-specific CD8+ T cells preferentially reside in the bone marrow.

A chromatic explosion: the development and future of multiparameter flow cytometry.

Increased IL-15 production is associated with higher susceptibility of memory CD4 T cells to simian immunodeficiency virus during acute infection.

Replication-defective adenovirus vectors with multiple deletions do not induce measurable vector-specific T cells in human trials.

Public clonotype usage identifies protective Gag-specific CD8+ T cell responses in SIV infection.

Acute HIV infection: it takes more than guts.

HIV vaccines: can mucosal CD4 T cells be protected?

High frequencies of resting CD4+ T cells containing integrated viral DNA are found in rhesus macaques during acute lentivirus infections.

Flow cytometry and the future of vaccine development.

Differential association of programmed death-1 and CD57 with ex vivo survival of CD8+ T cells in HIV infection.

Alpha4(+)beta7(hi)CD4(+) memory T cells harbor most Th-17 cells and are preferentially infected during acute SIV infection.

Differential specificity and immunogenicity of adenovirus type 5 neutralizing antibodies elicited by natural infection or immunization.

CD4 T cell subsets in the mucosa are CD28+Ki-67-HLA-DR-CD69+ but show differential infection based on alpha4beta7 receptor expression during acute SIV infection.

Autocrine production of beta-chemokines protects CMV-Specific CD4 T cells from HIV infection.

Trafficking, persistence, and activation state of adoptively transferred allogeneic and autologous Simian Immunodeficiency Virus-specific CD8(+) T cell clones during acute and chronic infection of rhesus macaques.

Distribution, persistence, and efficacy of adoptively transferred central and effector memory-derived autologous simian immunodeficiency virus-specific CD8+ T cell clones in rhesus macaques during acute infection.

Immunologic and virologic events in early HIV infection predict subsequent rate of progression.

The use of quantum dot nanocrystals in multicolor flow cytometry.

Priming immunization with DNA augments immunogenicity of recombinant adenoviral vectors for both HIV-1 specific antibody and T-cell responses.

Phenotypic and functional profile of HIV-inhibitory CD8 T cells elicited by natural infection and heterologous prime/boost vaccination.

Demonstration of cross-protective vaccine immunity against an emerging pathogenic Ebolavirus Species.

IL-10 production differentially influences the magnitude, quality, and protective capacity of Th1 responses depending on the vaccine platform.

Amine-reactive dyes for dead cell discrimination in fixed samples.

Data analysis in flow cytometry: the future just started.

Good cell, bad cell: flow cytometry reveals T-cell subsets important in HIV disease.

Rational design of envelope identifies broadly neutralizing human monoclonal antibodies to HIV-1.

Transient and persistent effects of IL-15 on lymphocyte homeostasis in nonhuman primates.

Vector choice determines immunogenicity and potency of genetic vaccines against Angola Marburg virus in nonhuman primates.

Publication of optimized multicolor immunofluorescence panels.

OMIP-001: Quality and phenotype of Ag-responsive human T-cells.

OMIP-002: Phenotypic analysis of specific human CD8+ T-cells using peptide-MHC class I multimers for any of four epitopes.

Genetic immunization in the lung induces potent local and systemic immune responses.

Heavy metal contaminants can eliminate quantum dot fluorescence.

Rapid SIV Env-specific mucosal and serum antibody induction augments cellular immunity in protecting immunized, elite-controller macaques against high dose heterologous SIV challenge.

SPICE: exploration and analysis of post-cytometric complex multivariate datasets.

Recombinant adenovirus serotype 26 (Ad26) and Ad35 vaccine vectors bypass immunity to Ad5 and protect nonhuman primates against ebolavirus challenge.

Safety (toxicity), pharmacokinetics, immunogenicity, and impact on elements of the normal immune system of recombinant human IL-15 in rhesus macaques.

Single-cell gene-expression profiling reveals qualitatively distinct CD8 T cells elicited by different gene-based vaccines.

High-throughput quantitative analysis of HIV-1 and SIV-specific ADCC-mediating antibody responses.

Focused evolution of HIV-1 neutralizing antibodies revealed by structures and deep sequencing.

CD8+ cellular immunity mediates rAd5 vaccine protection against Ebola virus infection of nonhuman primates.

A human memory T cell subset with stem cell-like properties.

The simian immunodeficiency virus targets central cell cycle functions through transcriptional repression in vivo.

Cytometry: today's technology and tomorrow's horizons.

Virus inhibition activity of effector memory CD8(+) T cells determines simian immunodeficiency virus load in vaccinated monkeys after vaccine breakthrough infection.

OMIP-005: Quality and phenotype of antigen-responsive rhesus macaque T cells.

OMIP-009: Characterization of antigen-specific human T-cells.

A deep profiler's guide to cytometry.

Brilliant violet fluorophores: a new class of ultrabright fluorescent compounds for immunofluorescence experiments.

Priming T-cell responses with recombinant measles vaccine vector in a heterologous prime-boost setting in non-human primates.

HIV-1 neutralizing antibodies display dual recognition of the primary and coreceptor binding sites and preferential binding to fully cleaved envelope glycoproteins.

CD4 T follicular helper cell dynamics during SIV infection.

Optimization and qualification of an 8-color intracellular cytokine staining assay for quantifying T cell responses in rhesus macaques for pre-clinical vaccine studies.

Increased memory differentiation is associated with decreased polyfunctionality for HIV but not for cytomegalovirus-specific CD8+ T cells.

OMIP-013: differentiation of human T-cells.

Quality assurance for polychromatic flow cytometry using a suite of calibration beads.

OMIP-015: human regulatory and activated T-cells without intracellular staining.

Early immunologic and virologic predictors of clinical HIV-1 disease progression.

Identification, isolation and in vitro expansion of human and nonhuman primate T stem cell memory cells.

Data exploration, quality control and testing in single-cell qPCR-based gene expression experiments.

Superior T memory stem cell persistence supports long-lived T cell memory.

Quantifying spillover spreading for comparing instrument performance and aiding in multicolor panel design.

Comparative analysis of the magnitude, quality, phenotype, and protective capacity of simian immunodeficiency virus gag-specific CD8+ T cells following human-, simian-, and chimpanzee-derived recombinant adenoviral vector immunization.

OMIP-017: human CD4(+) helper T-cell subsets including follicular helper cells.

Therapeutic vaccination expands and improves the function of the HIV-specific memory T-cell repertoire.

Highly multiplexed quantitation of gene expression on single cells.

DNA vaccine delivered by a needle-free injection device improves potency of priming for antibody and CD8+ T-cell responses after rAd5 boost in a randomized clinical trial.

Mixture models for single-cell assays with applications to vaccine studies.

OMIP-019: quantification of human γδT-cells, iNKT-cells, and hematopoietic precursors.

Protection against malaria by intravenous immunization with a nonreplicating sporozoite vaccine.

Safety and tolerability of a live oral Salmonella typhimurium vaccine candidate in SIV-infected nonhuman primates.

Differential impact of magnitude, polyfunctional capacity, and specificity of HIV-specific CD8+ T cell responses on HIV set point.

Immunological and virological mechanisms of vaccine-mediated protection against SIV and HIV.

Loss of circulating CD4 T cells with B cell helper function during chronic HIV infection.

Phase I randomized clinical trial of VRC DNA and rAd5 HIV-1 vaccine delivery by intramuscular (i.m.), subcutaneous (s.c.) and intradermal (i.d.) administration (VRC 011).

Establishment and maintenance of a PBMC repository for functional cellular studies in support of clinical vaccine trials.

Aerosol vaccination with AERAS-402 elicits robust cellular immune responses in the lungs of rhesus macaques but fails to protect against high-dose Mycobacterium tuberculosis challenge.

Enhanced potency of a broadly neutralizing HIV-1 antibody in vitro improves protection against lentiviral infection in vivo.

Chimpanzee adenovirus vaccine generates acute and durable protective immunity against ebolavirus challenge.

Homologous boosting with adenoviral serotype 5 HIV vaccine (rAd5) vector can boost antibody responses despite preexisting vector-specific immunity in a randomized phase I clinical trial.

Q and B values are critical measurements required for inter-instrument standardization and development of multicolor flow cytometry staining panels.

Redistribution, hyperproliferation, activation of natural killer cells and CD8 T cells, and cytokine production during first-in-human clinical trial of recombinant human interleukin-15 in patients with cancer.

A nonhuman primate toxicology and immunogenicity study evaluating aerosol delivery of AERAS-402/Ad35 vaccine: Evidence for transient t cell responses in peripheral blood and robust sustained responses in the lungs.

Safety and immunogenicity of Ebola virus and Marburg virus glycoprotein DNA vaccines assessed separately and concomitantly in healthy Ugandan adults: a phase 1b, randomised, double-blind, placebo-controlled clinical trial.

Antigen expression determines adenoviral vaccine potency independent of IFN and STING signaling.

Filarial infection modulates the immune response to Mycobacterium tuberculosis through expansion of CD4+ IL-4 memory T cells.

The genetic architecture of the human immune system: a bioresource for autoimmunity and disease pathogenesis.

A proposal for unified flow cytometer parameter naming.

Vaccine-Induced Linear Epitope-Specific Antibodies to Simian Immunodeficiency Virus SIVmac239 Envelope Are Distinct from Those Induced to the Human Immunodeficiency Virus Type 1 Envelope in Nonhuman Primates.

OMIP-029: Human NK-cell phenotypization.

Parsimonious Determination of the Optimal Infectious Dose of a Pathogen for Nonhuman Primate Models.

Breakthrough Virus Neutralization Resistance as a Correlate of Protection in a Nonhuman Primate Heterologous Simian Immunodeficiency Virus Vaccine Challenge Study.

HISTORY OF SCIENCE. Flow cytometry strikes gold.

Distributions of autofluorescence after compensation: Be panglossian, fret not.

Potential To Streamline Heterologous DNA Prime and NYVAC/Protein Boost HIV Vaccine Regimens in Rhesus Macaques by Employing Improved Antigens.

flowClean: Automated identification and removal of fluorescence anomalies in flow cytometry data.

Targeted Isolation of Antibodies Directed against Major Sites of SIV Env Vulnerability.

Targeting HIV-1 Env gp140 to LOX-1 Elicits Immune Responses in Rhesus Macaques.

Protection against malaria at 1 year and immune correlates following PfSPZ vaccination.

Adjuvant-dependent innate and adaptive immune signatures of risk of SIVmac251 acquisition.

Vaccine-Induced Antibodies that Neutralize Group 1 and Group 2 Influenza A Viruses.

Boosting of ALVAC-SIV Vaccine-Primed Macaques with the CD4-SIVgp120 Fusion Protein Elicits Antibodies to V2 Associated with a Decreased Risk of SIVmac251 Acquisition.

A simple tube adapter to expedite and automate thawing of viably frozen cells.

Rapid development of a DNA vaccine for Zika virus.

Highly Multiplexed, Single Cell Transcriptomic Analysis of T-Cells by Microfluidic PCR.

Reconstitution of Peripheral T Cells by Tissue-Derived CCR4+ Central Memory Cells Following HIV-1 Antiretroviral Therapy.

Unique cellular and humoral immunogenicity profiles generated by aerosol, intranasal, or parenteral vaccination in rhesus macaques.

Innate and adaptive immune traits are differentially affected by genetic and environmental factors.

Superiority in Rhesus Macaques of Targeting HIV-1 Env gp140 to CD40 versus LOX-1 in Combination with Replication-Competent NYVAC-KC for Induction of Env-Specific Antibody and T Cell Responses.

Attenuated PfSPZ Vaccine induces strain-transcending T cells and durable protection against heterologous controlled human malaria infection.

Combined single-cell quantitation of host and SIV genes and proteins ex vivo reveals host-pathogen interactions in individual cells.

Trispecific broadly neutralizing HIV antibodies mediate potent SHIV protection in macaques.

Geographic clonal tracking in macaques provides insights into HSPC migration and differentiation.

Toward Tuberculosis Vaccine Development: Recommendations for Nonhuman Primate Study Design.

Intranasal Live Influenza Vaccine Priming Elicits Localized B Cell Responses in Mediastinal Lymph Nodes.

The early expansion of anergic NKG2A/CD56/CD16 natural killer represents a therapeutic target in haploidentical hematopoietic stem cell transplantation.

A genetic IFN/STAT1/FAS axis determines CD4 T stem cell memory levels and apoptosis in healthy controls and Adult T-cell Leukemia patients.

HIV vaccine candidate activation of hypoxia and the inflammasome in CD14 monocytes is associated with a decreased risk of SIV acquisition.

Select gp120 V2 domain specific antibodies derived from HIV and SIV infection and vaccination inhibit gp120 binding to α4β7.