severe-burn-injury-in-a-swine-model-for-clinical-dressing-assessment

Severe Burn Injury in a Swine Model for Clinical Dressing Assessment

Wound healing is a dynamic repair process and is the most complex biological process in human life. In response to burn injury, alterations in biological ...

National Defense Medical Center

Cartilage is exposed to compression forces during joint loading. Therefore, exogenous stimuli are frequently used in cartilage tissue engineering strategies to enhance chondrocyte differentiation and extracellular matrix (ECM) secretion. In this study, human adipose-derived stem cells were seeded on a gelatin/polycaprolactone scaffold to evaluate the histochemical and functional improvement of tissue-engineered cartilage after hyperbaric oxygen/air treatment in a rabbit articular defect model. Behavior tests showed beneficial effects on weight-bearing and rear leg-supporting capacities after treatment of tissue-engineered cartilage with 2.5 ATA oxygen or air. Moreover, positron emission tomography images and immunohistochemistry staining demonstrated hydroxyapatite formation and increased ECM synthesis, respectively, at the tissue-engineered cartilage graft site after high pressure oxygen/air treatment. Based on these results, we concluded that hyperbaric oxygen and air treatment can improve the quality of tissue-engineered cartilage in vivo by increasing the synthesis of ECM. 

Histochemical and functional improvement of adipose-derived stem cell-based tissue-engineered cartilage by hyperbaric oxygen/air treatment in a rabbit articular defect model.

The pathogenesis of ketamine cystitis (KC) has been recently linked with immune response to patients but the same has not yet been established. Hence, this study aims to propose a possible immune mechanism of irreversible bladder damage caused by KC. A total of 53 KC patients and 21 healthy volunteers as controls have been retrospectively assessed. The levels of serum immunoglobulin E (IgE), IL-6, and IFN- of KC patients were significantly higher than those of controls, whereas the TGF- levels of KC patients substantially reduced but the IL-2 and IL-4 levels of KC patients were comparable to those of controls. Moreover, the KC patients had significantly higher counts of T1, T2, and T17 cells than those of controls. The immune response of KC users may begin with the IL-6 production and differentiation of T17 and may be followed by alternating between high expressions of T1 and T2. The IL-6 may further suppress the T cells which can aggravate chronic inflammation in KC patients and the imbalance in T17 and T cells may involve the pathogenesis of KC. Further investigation is needed to define the role of IL-6 in T1/T2/T17-regulated signaling pathway in ketamine-induced cystitis.

Gang-Yi Fan

Graduate Institute of Medical Sciences

National Defense Medical Center

Severe Burn Injury in a Swine Model for Clinical Dressing Assessment

Gang-Yi Fan

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National Defense Medical Center

Severe Burn Injury in a Swine Model for Clinical Dressing Assessment

Graduate Institute of Medical Sciences