Takaji Wakita

Processing of hepatitis C virus core protein is regulated by its C-terminal sequence.

Induction of hepatic injury by hepatitis C virus-specific CD8+ murine cytotoxic T lymphocytes in transgenic mice expressing the viral structural genes.

Inoculation of plasmids encoding Japanese encephalitis virus PrM-E proteins with colloidal gold elicits a protective immune response in BALB/c mice.

Repeated hepatocyte injury promotes hepatic tumorigenesis in hepatitis C virus transgenic mice.

Efficient replication of the genotype 2a hepatitis C virus subgenomic replicon.

Genotype 2a hepatitis C virus subgenomic replicon can replicate in HepG2 and IMY-N9 cells.

Regulation of hepatitis C virus replication by interferon regulatory factor 1.

Differences in hepatitis C virus core protein processing among genotypes 1 and 2.

Nonhepatic cell lines HeLa and 293 support efficient replication of the hepatitis C virus genotype 2a subgenomic replicon.

Nuclear localization of Japanese encephalitis virus core protein enhances viral replication.

Robust hepatitis C virus infection in vitro.

Production of infectious hepatitis C virus in tissue culture from a cloned viral genome.

Characterization of the E-138 (Glu/Lys) mutation in Japanese encephalitis virus by using a stable, full-length, infectious cDNA clone.

Comparison between subgenomic replicons of hepatitis C virus genotypes 2a (JFH-1) and 1b (Con1 NK5.1).

Robust production of infectious hepatitis C virus (HCV) from stably HCV cDNA-transfected human hepatoma cells.

Detection of anti-hepatitis C virus effects of interferon and ribavirin by a sensitive replicon system.

Production of infectious genotype 1a hepatitis C virus (Hutchinson strain) in cultured human hepatoma cells.

Subcellular localization of hepatitis C virus structural proteins in a cell culture system that efficiently replicates the virus.

[Production of infectious hepatitis C virus in cell culture].

Diverse effects of cyclosporine on hepatitis C virus strain replication.

Generation of infectious hepatitis C virus in immortalized human hepatocytes.

Hepatitis C virus entry depends on clathrin-mediated endocytosis.

Regulation of CXCL-8 (interleukin-8) induction by double-stranded RNA signaling pathways during hepatitis C virus infection.

Replication of a hepatitis C virus replicon clone in mouse cells.

E6AP ubiquitin ligase mediates ubiquitylation and degradation of hepatitis C virus core protein.

Upregulation of indoleamine 2,3-dioxygenase in hepatitis C virus infection.

Production of infectious hepatitis C virus of various genotypes in cell cultures.

Neutralization assays for echovirus 18 isolates in 2006.

CD81 expression is important for the permissiveness of Huh7 cell clones for heterogeneous hepatitis C virus infection.

Cell culture and infection system for hepatitis C virus.

An infectious and selectable full-length replicon system with hepatitis C virus JFH-1 strain.

The roles of CD81 and glycosaminoglycans in the adsorption and uptake of infectious HCV particles.

Infection of human hepatocyte chimeric mouse with genetically engineered hepatitis C virus and its susceptibility to interferon.

The NS3 helicase and NS5B-to-3'X regions are important for efficient hepatitis C virus strain JFH-1 replication in Huh7 cells.

Monitoring the antiviral effect of alpha interferon on individual cells.

An attenuated strain of enterovirus 71 belonging to genotype a showed a broad spectrum of antigenicity with attenuated neurovirulence in cynomolgus monkeys.

Rapid and real-time detection of hepatitis A virus by reverse transcription loop-mediated isothermal amplification assay.

Molecular biology of hepatitis C virus.

Robust production of infectious viral particles in Huh-7 cells by introducing mutations in hepatitis C virus structural proteins.

The lipid droplet is an important organelle for hepatitis C virus production.

Hepatitis C viral life cycle.

DDX3 DEAD-box RNA helicase is required for hepatitis C virus RNA replication.

HCV research and anti-HCV drug discovery: toward the next generation.

Development of plaque assays for hepatitis C virus-JFH1 strain and isolation of mutants with enhanced cytopathogenicity and replication capacity.

Scavenger receptor class B type I is a key host factor for hepatitis C virus infection required for an entry step closely linked to CD81.

Hepatitis C virus non-structural proteins responsible for suppression of the RIG-I/Cardif-induced interferon response.

Cooperative effect of the attenuation determinants derived from poliovirus sabin 1 strain is essential for attenuation of enterovirus 71 in the NOD/SCID mouse infection model.

Critical role of virion-associated cholesterol and sphingolipid in hepatitis C virus infection.

Apolipoprotein B-dependent hepatitis C virus secretion is inhibited by the grapefruit flavonoid naringenin.

Trans-encapsidation of hepatitis C virus subgenomic replicon RNA with viral structure proteins.

Intragenotypic JFH1 based recombinant hepatitis C virus produces high levels of infectious particles but causes increased cell death.

Interaction of hepatitis C virus nonstructural protein 5A with core protein is critical for the production of infectious virus particles.

Hepatitis C virus-infected hepatocytes extrinsically modulate dendritic cell maturation to activate T cells and natural killer cells.

Baculovirus mediated production of infectious hepatitis C virus in human hepatoma cells stably expressing T7 RNA polymerase.

Virological characterization of the hepatitis C virus JFH-1 strain in lymphocytic cell lines.

Griseofulvin, an oral antifungal agent, suppresses hepatitis C virus replication in vitro.

The DNA damage sensors ataxia-telangiectasia mutated kinase and checkpoint kinase 2 are required for hepatitis C virus RNA replication.

Induction of incomplete autophagic response by hepatitis C virus via the unfolded protein response.

Noroviruses distinguish between type 1 and type 2 histo-blood group antigens for binding.

Hepatitis C virus JFH-1 strain infection in chimpanzees is associated with low pathogenicity and emergence of an adaptive mutation.

Establishment of an infectious genotype 1b hepatitis C virus clone in human hepatocyte chimeric mice.

Characterization of pharmacologically active compounds that inhibit poliovirus and enterovirus 71 infectivity.

Hepatitis C virus infection sensitizes human hepatocytes to TRAIL-induced apoptosis in a caspase 9-dependent manner.

Saturation mutagenesis reveals that GLU54 of norovirus 3C-like protease is not essential for the proteolytic activity.

[Hepatitis C virus vaccine].

Characterization of infectious hepatitis C virus from liver-derived cell lines.

Efficient production of type 2 porcine circovirus-like particles by a recombinant baculovirus.

Isolation of JFH-1 strain and development of an HCV infection system.

Cellular vimentin content regulates the protein level of hepatitis C virus core protein and the hepatitis C virus production in cultured cells.

Development of a rapid high-throughput method for high-resolution melting analysis for routine detection and genotyping of noroviruses.

Proteasomal turnover of hepatitis C virus core protein is regulated by two distinct mechanisms: a ubiquitin-dependent mechanism and a ubiquitin-independent but PA28gamma-dependent mechanism.

Arsenic trioxide inhibits hepatitis C virus RNA replication through modulation of the glutathione redox system and oxidative stress.

Self-assembly of sapovirus recombinant virus-like particles from polyprotein in mammalian cells.

Hepatitis C virus infection of T cells inhibits proliferation and enhances fas-mediated apoptosis by down-regulating the expression of CD44 splicing variant 6.

Hepatitis C virus nonstructural 4B protein modulates sterol regulatory element-binding protein signaling via the AKT pathway.

Identification of annexin A1 as a novel substrate for E6AP-mediated ubiquitylation.

Involvement of creatine kinase B in hepatitis C virus genome replication through interaction with the viral NS4A protein.

Quantitative analysis of fecal sapovirus shedding: identification of nucleotide substitutions in the capsid protein during prolonged excretion.

Modification of hepatitis C virus 1b RNA polymerase to make a highly active JFH1-type polymerase by mutation of the thumb domain.

Double-stranded RNA-induced interferon-beta and inflammatory cytokine production modulated by hepatitis C virus serine proteases derived from patients with hepatic diseases.

Characterization of sapoviruses detected in gastroenteritis outbreaks and identification of asymptomatic adults with high viral load.

Cell culture-adaptive mutations in the NS5B gene of hepatitis C virus with delayed replication and reduced cytotoxicity.

Surveillance of pathogens in outpatients with gastroenteritis and characterization of sapovirus strains between 2002 and 2007 in Kumamoto Prefecture, Japan.

Cellular kinase inhibitors that suppress enterovirus replication have a conserved target in viral protein 3A similar to that of enviroxime.

Human P-selectin glycoprotein ligand-1 is a functional receptor for enterovirus 71.

Identification of bisindolylmaleimides and indolocarbazoles as inhibitors of HCV replication by tube-capture-RT-PCR.

Distinct genotype and antigenicity among genogroup II sapoviruses.

Characterization of anti-idiotypic antibodies mimicking antibody- and receptor-binding sites on hepatitis A virus.

An in-house-anti-hepatitis A virus (HAV)-specific immunoglobulin M capture enzyme-linked immunosorbent assay: evaluation and application to an HAV outbreak.

Knockdown of autophagy-related gene decreases the production of infectious hepatitis C virus particles.

Efficient replication systems for hepatitis C virus using a new human hepatoma cell line.

Structural and biological constraints on diversity of regions immediately upstream of cleavage sites in calicivirus precursor proteins.

HCV genotype 1b chimeric replicon with NS5B of JFH-1 exhibited resistance to cyclosporine A.

Requirement of cellular DDX3 for hepatitis C virus replication is unrelated to its interaction with the viral core protein.

Evaluation of hepatitis C virus core antigen assays in detecting recombinant viral antigens of various genotypes.

Absence of viral interference and different susceptibility to interferon between hepatitis B virus and hepatitis C virus in human hepatocyte chimeric mice.

Ethanol enhances hepatitis C virus replication through lipid metabolism and elevated NADH/NAD+.

Systematic identification of microRNA and messenger RNA profiles in hepatitis C virus-infected human hepatoma cells.

Development of a reverse transcription-loop-mediated isothermal amplification (RT-LAMP) system for a highly sensitive detection of enterovirus in the stool samples of acute flaccid paralysis cases.

Identification of hepatitis C virus genotype 2a replicon variants with reduced susceptibility to ribavirin.

2',5'-Oligoadenylate synthetase-like gene highly induced by hepatitis C virus infection in human liver is inhibitory to viral replication in vitro.

Impaired antiviral activity of interferon alpha against hepatitis C virus 2a in Huh-7 cells with a defective Jak-Stat pathway.

Molecular characterization of Sapovirus detected in a gastroenteritis outbreak at a wedding hall.

Production of infectious hepatitis C virus by using RNA polymerase I-mediated transcription.

Toll-like receptor 2 senses hepatitis C virus core protein but not infectious viral particles.

Biological properties of purified recombinant HCV particles with an epitope-tagged envelope.

RNA polymerase activity and specific RNA structure are required for efficient HCV replication in cultured cells.

Hepatitis C virus controls interferon production through PKR activation.

La protein required for internal ribosome entry site-directed translation is a potential therapeutic target for hepatitis C virus replication.

Primary hepatocyte culture supports hepatitis C virus replication: a model for infection-associated hepatocarcinogenesis.

Development of a particle agglutination method with soluble virus receptor for identification of poliovirus.

E6AP ubiquitin ligase mediates ubiquitin-dependent degradation of peroxiredoxin 1.

A disulfide-bonded dimer of the core protein of hepatitis C virus is important for virus-like particle production.

Production of infectious hepatitis C virus in primary cultures of human adult hepatocytes.

Cell culture and in vivo analyses of cytopathic hepatitis C virus mutants.

A bifunctional anti-enterovirus compound that inhibits replication and the early stage of enterovirus 71 infection.

Structure of hepatitis E virion-sized particle reveals an RNA-dependent viral assembly pathway.

Hepatitis C virus NS2 protein triggers endoplasmic reticulum stress and suppresses its own viral replication.

Inhibitory effects on HAV IRES-mediated translation and replication by a combination of amantadine and interferon-alpha.

Infectivity of hepatitis C virus is influenced by association with apolipoprotein E isoforms.

Sphingomyelin activates hepatitis C virus RNA polymerase in a genotype-specific manner.

Adaptive mutations in the genomes of enterovirus 71 strains following infection of mouse cells expressing human P-selectin glycoprotein ligand-1.

Tyrosine sulfation of the amino terminus of PSGL-1 is critical for enterovirus 71 infection.

Chaperonin TRiC/CCT participates in replication of hepatitis C virus genome via interaction with the viral NS5B protein.

Phosphatidylinositol 4-kinase III beta is a target of enviroxime-like compounds for antipoliovirus activity.

Comparative site-directed mutagenesis in the catalytic amino acid triad in calicivirus proteases.

The ESCRT system is required for hepatitis C virus production.

Studies on virus kinetics using infectious fluorescence-tagged hepatitis C virus cell culture.

Bioluminescence technologies to detect calicivirus protease activity in cell-free system and in infected cells.

Mechanism of action of ribavirin in a novel hepatitis C virus replication cell system.

Hepatitis C virus RNA replication in human stellate cells regulates gene expression of extracellular matrix-related molecules.

Malnutrition impairs interferon signaling through mTOR and FoxO pathways in patients with chronic hepatitis C.

In vivo adaptation of hepatitis C virus in chimpanzees for efficient virus production and evasion of apoptosis.

Production and characterization of HCV particles from serum-free culture.

Expression of human factors CD81, claudin-1, scavenger receptor, and occludin in mouse hepatocytes does not confer susceptibility to HCV entry.

Structural requirements of virion-associated cholesterol for infectivity, buoyant density and apolipoprotein association of hepatitis C virus.

Will there be an HCV meeting in 2020? Summary of the 17th international meeting on hepatitis C virus and related viruses.

Anti-ulcer agent teprenone inhibits hepatitis C virus replication: potential treatment for hepatitis C.

Development of recombinant hepatitis C virus with NS5A from strains of genotypes 1 and 2.

Hepatitis C Virus nonstructural 5A protein inhibits lipopolysaccharide-mediated apoptosis of hepatocytes by decreasing expression of Toll-like receptor 4.

Characterization of self-assembled virus-like particles of rat hepatitis E virus generated by recombinant baculoviruses.

Role of the endoplasmic reticulum-associated degradation (ERAD) pathway in degradation of hepatitis C virus envelope proteins and production of virus particles.

Development of a poliovirus neutralization test with poliovirus pseudovirus for measurement of neutralizing antibody titer in human serum.

Generation of a recombinant reporter hepatitis C virus useful for the analyses of virus entry, intra-cellular replication and virion production.

Hepatitis C virus reveals a novel early control in acute immune response.

Genetic variation of the IL-28B promoter affecting gene expression.

Human sapovirus classification based on complete capsid nucleotide sequences.

Potent and selective inhibition of hepatitis C virus replication by novel phenanthridinone derivatives.

[Critical roles of lipids in the HCV life cycle].

[Hepatitis C virus culture and its application].

Possible widespread presence of hepatitis A virus subgenotype IIIA in Japan: Recent trend of hepatitis A causing acute liver failure.

Production of Infectious Chimeric Hepatitis C Virus Genotype 2b Harboring Minimal Regions of JFH-1.

Epidemiological and genetic analyses of a diffuse outbreak of hepatitis A in Japan, 2010.

Detergent-induced activation of the hepatitis C virus genotype 1b RNA polymerase.

Replication and infectivity of a novel genotype 1b hepatitis C virus clone.