ribo-seq-bacteria-sample-collection-library-preparation-protocol-for

RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing

The ribosome profiling technique (RIBO-seq) is currently the most effective tool for studying the process of protein synthesis in vivo. The advantage of ...

Maria Curie-Sklodowska University

Fourier Transform Infrared (FT-IR) spectroscopy and imaging combined with hierarchical cluster analysis (HCA) was applied to analyse biochemical properties of Early Middle Ages hemp ( L.) bast fibres collected from lake bottom sediment of lake Słone. The examined plant macrofossil material constitutes residues of the hemp retting process that took place in the 7th-8th century. By comparison of three samples: untreated isolated bast fibres, and fibres incubated overnight at 4 and 37 °C, we were able to mimic the retting conditions. Using FT-IR qualitative and semi-quantitative assessment of the primary polysaccharides content, total protein content, and their spatial distribution was performed within the hemp fibres. The concentration of cellulose remained vastly unchanged, while the concentration of lignin and pectin was the highest in the untreated sample. The spatial distributions of compounds were heterogeneous in the untreated and 4 °C-incubated samples, and homogenous in the specimen processed at 37 °C. Interestingly, a higher amide content was detected in the latter sample indicating the highest degree of enzymatic degradation. In this study, we show that the spectroscopic methods allow for a non-destructive evaluation of biochemical composition of plant fibres without preparation, which can be an appropriate approach for studying ancient plant remains.

Olga Chrobak

Laboratory of Gene Expression,
ECOTECH-COMPLEX,
Department of Molecular Biology,
Institute of Biological Sciences,
Laboratory of Gene Expression, ECOTECH-Complex,
Department of Molecular Biology, Institute of Biological Sciences

Maria Curie-Sklodowska University

RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing