embryo-transfer-surgery-via-laparotomy-in-gilts

Embryo Transfer Surgery via Laparotomy in Gilts

This protocol aims to demonstrate the surgical technique for transferring cloned pig embryos to the oviduct, a method widely used in the production of ...

Universidade de São Paulo

Increasing evidence suggests that environmental exposures can modify epigenetic marks in the germline, leading to the transmission of abnormal post-fertilization sperm epigenetic indicators and affecting embryonic development. Given the pivotal role of sperm cells in determining embryo quality, there is growing interest in understanding the potential effects of sperm sex sorting on embryo quality. This study aimed to investigate the impact of bovine sperm sexing on in vitro embryo production (IVP) and to associate molecular aspects of embryos analysis. Frozen semen samples from five Nellore bulls were used, with each bull contributing unsexed sperm (conventional semen - CV treatment) and female and male sexed sperm pooled after thawing (SX treatment). First, semen quality was assessed, including motility, morphology, acrosome integrity, and chromatin integrity to denaturation. Then, IVP was carried out, focusing on embryonic production and developmental kinetics. In the third experiment, embryo quality was evaluated by examining the gene expression of key markers (OCT4, NANOG, DNMT3A, TET1, and Fematrin-1) and the methylation pattern of the Satellite-1 and α-Satellite genes in blastocysts. Differences between CV and SX semen were only observed in motility, which was lower in SX compared with CV (P < 0.05). Although cleavage was similar, the SX groups showed lower blastocyst production than CV (P < 0.05). Of the genes evaluated, only NANOG showed high expression in the CV blastocysts compared with the SX blastocysts, but the methylation pattern revealed no differences. In conclusion, sex sorting markedly affects sperm motility and in vitro embryo production but showed no significant impact on embryo quality.

Ligiane Leme

Centro de Estudos sobre o Genoma Humano e Células-Tronco,
Instituto de Biociências,
Centro de Estudos sobre o Genoma Humano e Células-Tronco, Instituto de Biociências

Universidade de São Paulo

Embryo Transfer Surgery via Laparotomy in Gilts

Ligiane Leme

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Centro de Estudos sobre o Genoma Humano e Células-Tronco,Instituto de Biociências,Centro de Estudos sobre o Genoma Humano e Células-Tronco, Instituto de Biociências

Universidade de São Paulo

Embryo Transfer Surgery via Laparotomy in Gilts

Centro de Estudos sobre o Genoma Humano e Células-Tronco,
Instituto de Biociências,
Centro de Estudos sobre o Genoma Humano e Células-Tronco, Instituto de Biociências