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Novel Triple-Loop Technique for Suturing TFCC Injuries without Transosseous Tunnel

Injuries to the triangular fibrocartilage complex (TFCC) are the most common cause of ulnar-sided wrist pain. As deepening of the research on the function ...

Shaoxing People's Hospital

Rheumatoid arthritis, a disabling autoimmune disease, is associated with altered gene expression in circulating immune cells and synovial tissues. Accumulating evidence has suggested that long non-coding RNAs (lncRNAs), which modulate gene expression through multiple mechanisms, are important molecules involved in immune and inflammatory pathways. Importantly, many studies have reported that lncRNAs can be utilized as biomarkers for disease diagnosis and prognostication. Recently, dysregulation of lncRNAs in rheumatoid arthritis and other autoimmune diseases has been revealed. Experimental studies also confirmed their crosstalk with matrix metalloproteinases, nuclear factor-κB signalling and T-cell response pertinent to autoimmunity and inflammation. Circulating lncRNAs, such as HOTAIR, differentiated patients with rheumatoid arthritis from healthy subjects. Taken together, lncRNAs are good candidates as biomarkers and therapeutic targets in rheumatoid arthritis. Further investigation on in vivo delivery of these regulatory molecules and large-cohort validation of their clinical applicability may be useful.

Long non-coding RNAs in rheumatoid arthritis.

Steroid-induced osteonecrosis of the femoral head (ONFH) is a common orthopaedic disease of which early detection remains clinically challenging. Accumulating evidences indicated that circulating microRNAs (miRNAs) plays vital roles in the development of several bone diseases. However, the association between circulating miRNAs and steroid-induced ONFH remains elusive.

Circulating microRNA signature of steroid-induced osteonecrosis of the femoral head.

Steroid-induced osteonecrosis of the femoral head (ONFH) is a common orthopedic disease. The lack of specific manifestations and effective diagnostic methods make it difficult for this disease to be diagnosed at early stages. Recent studies have shown that microRNAs (miRNA) participate in the development of steroid-induced ONFH, but there is limited research into the diagnostic use of circulating miRNAs. Blood samples from 23 human subjects (7 systemic lupus erythematosus (SLE) patients with steroid-induced ONFH; 7 SLE controls without ONFH; and 9 healthy controls) and 71 rats (19 with steroid-induced ONFH; 28 receiving steroids without ONFH; and 24 untreated controls) were collected to verify the abundance of changes of 6 previously identified ONFH-associated plasma miRNAs (miR-423-5p, miR-99a-5p, miR-10a-5p, miR-21-5p, miR-130a-3p and miR-6787-5p) by quantitative RT-PCR (Reverse Transcription-Polymerase Chain Reaction). In humans, the circulating levels of miR-10a-5p, miR-99a-5p and miR-21-5p were increased in SLE patients treated with cortico steroid regardless of ONFH status when compared with healthy controls. However, miR-423-5p, miR-6787-5p and miR-130a-3p showed no significant differences between the three groups. In the rat model, the success rate of steroid-induced ONFH was 40.4% (19/47) based on pathological examination and confirmation by micro-CT scan. Similar to human plasma, the circulating levels of miR-10a-5p, miR-99a-5p and miR-21-5p were increased in steroid-treated rats independent of ONFH development. The serum levels of miR-10a-5p, miR-99a-5p and miR-21-5p were increased by steroid treatment regardless of ONFH development in both humans and rats. These data suggested that miR-10a-5p, miR-99a-5p and miR-21-5p are steroid-responsive circulating miRNAs, but they are not specific for diagnosing steroid-induced ONFH.

miR-10a-5p, miR-99a-5p and miR-21-5p are steroid-responsive circulating microRNAs.

Spinal cord injury (SCI) is a devastating condition, often leading to significant disability and impairment. As crucial immune cells, macrophages play a critical role in the pathophysiology of SCI. Understanding the current state of knowledge and research trends related to macrophages in SCI is crucial for developing effective therapeutic interventions.

Knowledge Mapping of Macrophage in Spinal Cord Injury: A Bibliometric Analysis.

This review offered a comprehensive analysis of tendon and ligament injuries, emphasizing the crucial role of tendon-derived stem cells (TDSCs) in tissue engineering as a potential solution for these challenging medical conditions. Tendon and ligament injuries, prevalent among athletes, the elderly, and laborers, often result in long-term disability and reduced quality of life due to the poor intrinsic healing capacity of these avascular structures. The formation of biomechanically inferior scar tissue and a high rate of reinjury underscore the need for innovative approaches to enhance and guide the regenerative process. This review delved into the complexities of tendon and ligament structure and function, types of injuries and their impacts, and the limitations of the natural repair process. It particularly focused on the role of TDSCs within the context of tissue engineering. TDSCs, with their ability to differentiate into tenocytes, are explored in various applications, including biocompatible scaffolds for cell tracking, co-culture systems to optimize tendon-bone healing, and graft healing techniques. The review also addressed the challenges of immunoreactivity post-transplantation, the importance of pre-treating TDSCs, and the potential of hydrogels and decellularized matrices in supporting tendon regeneration. It concluded by highlighting the essential roles of mechanical and molecular stimuli in TDSC differentiation and the current challenges in the field, paving the way for future research directions.

Role of tendon-derived stem cells in tendon and ligament repair: focus on tissue engineer.

Osteoarthritis (OA) is a chronic joint disease characterized by cartilage degeneration. In this study, we developed hydrogel microspheres for cartilage repair in OA. We designed and synthesized a novel nanomaterial using liposomes (Lipo), which exhibits cartilage-targeting ability and pH responsiveness. These liposomes were surface-functionalized with cartilage-targeting peptides (WYRGRL), encapsulated with the cationic lipid 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP), incorporated with a pH-responsive hydrazone (HyD) bond, and loaded with the bioactive molecule kartogenin (KGN). These novel liposomes were embedded within methacrylated gelatin (GM) hydrogel microspheres to create the WYRGRL-DOTAP-Lipo@KGN@GM (WDLKG) hydrogel microspheres. Characterization tests revealed that the novel liposomes WYRGRL-DOTAP-Lipo@KGN (WDLK) exhibited cartilage-targeting ability and pH responsiveness and the WDLKG hydrogel microspheres showed excellent dispersibility and uniform size. Further in vitro cell-experiments demonstrated that WDLKG hydrogel microspheres effectively promoted chondrogenesis, and exhibited anti-inflammatory effects via enhancing the anabolism while reducing the catabolism in chondrocytes. Transcriptomic analysis revealed that WDLKG induced chondrogenesis primarily through collagen trimer formation and collagen-containing extracellular matrix biosynthesis. In vivo experiments in a mouse OA model confirmed the sustained release of WDLKG and demonstrated that intra-articular injection of WDLKG significantly inhibited cartilage degeneration. These findings underscore the potential of our nanomaterials for targeted therapy, advancing OA cartilage treatment.

Enhanced osteoarthritis treatment using an injectable pH-responsive and cartilage-targeted liposome-anchored kartogenin-incorporated methacrylated gelatin hydrogel microspheres.

Chao Jiang

Department of Orthopedics

Shaoxing People's Hospital

Novel Triple-Loop Technique for Suturing TFCC Injuries without Transosseous Tunnel

Chao Jiang

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Department of Orthopedics

Shaoxing People's Hospital

Novel Triple-Loop Technique for Suturing TFCC Injuries without Transosseous Tunnel

Department of Orthopedics