Core - Cell Biology

Chapter 32

Analyzing Cells and Proteins

32.1 : Overview Of Cell Separation And Isolation

32.1 : Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, ...

32.2 : Cell Culture

32.2 : Cell Culture

Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are ...

32.3 : Cell Lines

32.3 : Cell Lines

A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number ...

32.4 : Hybridoma Technology

32.4 : Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or ...

32.5 : Tissue Homogenization and Cell Lysis

32.5 : Tissue Homogenization and Cell Lysis

Tissue homogenization involves disintegrating tissue architecture and lysing cells, and is an early step in isolating and analyzing cellular components. ...

32.6 : Subcellular Fractionation

32.6 : Subcellular Fractionation

The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular ...

32.7 : Flow Cytometry

32.7 : Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing ...

32.8 : Principles Of Column Chromatography

32.8 : Principles Of Column Chromatography

The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. ...

32.9 : Types Of Column Chromatography

32.9 : Types Of Column Chromatography

The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating ...

32.10 : Immunoprecipitation

32.10 : Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native ...

32.11 : Tagging and Fusion Proteins

32.11 : Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from ...

32.12 : SDS-PAGE

32.12 : SDS-PAGE

Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under ...

32.13 : Western Blotting

32.13 : Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting ...

32.14 : Two-dimensional Gel Electrophoresis

32.14 : Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method ...

32.15 : Enzyme-Linked Immunosorbent Assay

32.15 : Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays ...

32.16 : MALDI-TOF Mass Spectrometry

32.16 : MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological ...

32.17 : Peptide Identification Using Tandem Mass Spectrometry

32.17 : Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass ...

$32.18 : X-ray Diffraction of Biological Samples$ 01:10

32.18 : X-ray Diffraction of Biological Samples

X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, ...

32.19 : Applications Of NMR In Biology

32.19 : Applications Of NMR In Biology

Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an ...

32.20 : Proteomics

32.20 : Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, ...

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