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Core - Cell Biology

    Chapter 33

    Visualizing Cells, Tissues, and Molecules

    33.1 : Imaging Biological Samples with Optical Microscopy01:18

    33.1 : Imaging Biological Samples with Optical Microscopy

    Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in ...

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    33.2 : Phase Contrast and Differential Interference Contrast Microscopy01:26

    33.2 : Phase Contrast and Differential Interference Contrast Microscopy

    Phase-Contrast Microscopes In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular ...

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    33.3 : Fixation and Sectioning01:03

    33.3 : Fixation and Sectioning

    Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens. The simplest type of preparation ...

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    33.4 : Immunofluorescence Microscopy01:12

    33.4 : Immunofluorescence Microscopy

    A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as ...

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    33.5 : Immunocytochemistry and Immunohistochemistry01:22

    33.5 : Immunocytochemistry and Immunohistochemistry

    Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The ...

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    33.6 : Confocal Fluorescence Microscopy01:16

    33.6 : Confocal Fluorescence Microscopy

    Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability ...

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    33.7 : Protein Dynamics in Living Cells01:19

    33.7 : Protein Dynamics in Living Cells

    Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET. Fluorescent ...

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    33.8 : Total Internal Reflection Fluorescence Microscopy01:05

    33.8 : Total Internal Reflection Fluorescence Microscopy

    Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid ...

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    33.9 : Atomic Force Microscopy01:08

    33.9 : Atomic Force Microscopy

    Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, ...

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    33.10 : Super-resolution Fluorescence Microscopy01:37

    33.10 : Super-resolution Fluorescence Microscopy

    Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread ...

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    33.11 : Overview of Electron Microscopy01:25

    33.11 : Overview of Electron Microscopy

    The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can ...

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    33.12 : Scanning Electron Microscopy01:07

    33.12 : Scanning Electron Microscopy

    A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a ...

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    33.13 : Transmission Electron Microscopy01:15

    33.13 : Transmission Electron Microscopy

    In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an ...

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    33.14 : Preparation of Samples for Electron Microscopy01:20

    33.14 : Preparation of Samples for Electron Microscopy

    To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does ...

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    33.15 : Immunogold Electron Microscopy01:20

    33.15 : Immunogold Electron Microscopy

    Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and ...

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    33.16 : Cryo-electron Microscopy01:28

    33.16 : Cryo-electron Microscopy

    Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological ...

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    33.17 : Electron Microscope Tomography and Single-particle Reconstruction01:07

    33.17 : Electron Microscope Tomography and Single-particle Reconstruction

    Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron ...

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