The 3D Culturing of Organoids from Murine Intestinal Crypts and a Single Stem Cell for Organoid ResearchYuta Takase 1, Kazuto Fujishima 2,3, Toshio Takahashi 1
1Suntory Foundation for Life Sciences, Bioorganic Research Institute, 2Institute for Integrated Cell-Material Sciences (KUIAS-iCeMS), Kyoto University, 3Faculty of Medicine, Osaka Medical and Pharmaceutical University
We describe a protocol to isolate murine small intestinal crypts and culture intestinal 3D organoids from the crypts. Additionally, we describe a method to generate organoids from a single intestinal stem cell in the absence of a sub-epithelial cellular niche.
