Studying the Epithelial Effects of Intestinal Inflammation In Vitro on Established Murine ColonoidsErin Crawford *1, Heather L. Mentrup *2,3, Elizabeth A. Novak 2,3, Kevin P. Mollen 2,3
1Division of Pediatric Gastroenterology, Hepatology and Nutrition, UPMC Children’s Hospital of Pittsburgh, 2Division of Pediatric Surgery, UPMC Children’s Hospital of Pittsburgh, 3Department of Surgery, University of Pittsburgh School of Medicine
We describe a protocol detailing the isolation of murine colonic crypts for the development of 3-dimensional colonoids. The established colonoids can then be terminally differentiated to reflect the cellular composition of the host epithelium prior to receiving an inflammatory challenge or being directed to establish an epithelial monolayer.
