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Univ-Lille, Inserm, CHU Lille, Institute Pasteur Lille, U1190 - EGID F-59000 Lille

1 ARTICLES PUBLISHED IN JoVE

Biology

Evaluation of the Impact of a New Cooling Cell Processor System on Islet Cell Isolation Facility

Naziha Menasria^1,2, Marten Engelse³, Julien Thévenet², Weam El Roz¹, Ayan Guled¹, Abdul-Badi Abou-Samra¹, François Pattou², Julie Kerr -Conte²

¹Qatar Metabolic Institute, Hamad Medical Corporation, ²Univ-Lille, Inserm, CHU Lille, Institute Pasteur Lille, U1190 - EGID F-59000 Lille, ³Department of Nephrology, Leiden University Medical Center (LUMC)

The protocol here describes a custom cooling cell processor system that is compatible with a good manufacturing practices (GMP) cleanroom to improve cell viability post-processing.