Dual Immunofluorescence of γH2AX and 53BP1 in Human Peripheral LymphocytesAurora Falaschi 1, Anna Chiaramonte 1,2, Serena Testi 1, Roberto Scarpato 1
1Unità di Genetica, Dipartimento di Biologia, University of Pisa, 2Department of Women-Child-Newborn Obstetrics and Gynaecology, Foundation IRCCS Ca' Granda Ospedale Maggiore Policlinico
This protocol presents a method to assess the formation and repair of DNA double-strand breaks through the simultaneous detection of γH2AX and 53BP1 foci in interphase nuclei of bleomycin-treated human peripheral lymphocytes.
