Chemical Affinity-Based Isolation of Extracellular Vesicles from Biofluids for Proteomics and Phosphoproteomics AnalysisYi-Kai Liu 1, Zhuojun Luo 1, Anton Iliuk 1,2, Weiguo Andy Tao 1,2,3,4
1Department of Biochemistry, Purdue University, 2Tymora Analytical Operations, 3Department of Chemistry, Purdue University, 4Purdue Institute for Cancer Research, Purdue University
The present protocol provides detailed descriptions for the efficient isolation of urinary extracellular vesicles utilizing functionalized magnetic beads. Moreover, it encompasses subsequent analyses, including western blotting, proteomics, and phosphoproteomics.
