The Combination of Mechanically Isolated Stromal Vascular Fraction and Fibrin Hydrogel: A Processing ProtocolPablo Pfister *1, Gregory Reid *2, Tabea Breckwoldt 2, Mauro Vasella 2, Ann-Kathrin Seitz 2, Seung Yong Song 3, Bong-Sung Kim 2
1Department of Surgery, Triemli City Hospital Zurich, 2Department of Plastic Surgery and Hand Surgery, University Hospital Zurich, University of Zurich, 3Institute for Human Tissue Restoration, Department of Plastic and Reconstructive Surgery, Yonsei University College of Medicine
Mechanically isolated stromal vascular fraction (SVF) in combination with a fibrin hydrogel offers an easy and efficient carrier for viable adipose-derived stromal cells for various indications, including tissue engineering and or wound healing purposes. Here, we present the preparation of a mechanical SVF (mSVF)-fibrin hydrogel construct for translational research and clinical application.
