Visualizing Low-Abundance Proteins and Post-Translational Modifications in Living Drosophila Embryos via Fluorescent Antibody InjectionQihong Zheng *1, Xiaoxiang Xiang *1, Yan Yan 2,3, Huapeng H. Yu 1
1School of Life Sciences, SUSTech University, 2Shenzhen PKU-HKUST Medical Center, 3Division of Life Science, Hong Kong University of Science and Technology
This protocol describes the customized antibody-based fluorescence labeling and injection into early Drosophila embryos to enable live imaging of low-abundance proteins or post-translational modifications that are challenging to detect using traditional GFP/mCherry-tag approaches.
