Analysis of Protein Complex Formation at Micromolar Concentrations by Coupling Microfluidics with Mass PhotometryMyndert Claasen 1, Zornitsa Kofinova 1, Matteo Contino 2, Weston B. Struwe 3,4
1Refeyn Ltd., 2Adaptix Ltd., 3Physical and Theoretical Chemistry Laboratory, Department of Chemistry, University of Oxford, 4The Kavli Institute for Nanoscience Discovery, University of Oxford
This protocol combines mass photometry with a novel microfluidics system to investigate low-affinity protein-protein interactions. This approach is based on the rapid dilution of highly concentrated complexes in solution, which enables low-affinity measurements and broadens the applicability of mass photometry.
